Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ...
In the present study, the cloning and sequence analysis of interleukin‐6 (IL‐6) from the giant panda ( Ailuropoda melanoleuca ) were described. Sequencing revealed that IL‐6 complementary DNA contains a 636 base‐pair open reading frame encoding 211 amino acids. Homology analyses indicated that the i...
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ftdatacite:10.6084/m9.figshare.c.1889264 2024-04-28T08:23:03+00:00 Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... Zeng, Bo Yue, Bisong Xu, Liu Peng, Rui Zou, Fangdong 2015 https://dx.doi.org/10.6084/m9.figshare.c.1889264 https://tandf.figshare.com/collections/Molecular_cloning_and_sequence_analysis_of_the_gene_encoding_interleukin_6_of_the_giant_panda_i_Ailuropoda_melanoleuca_i_/1889264 unknown Taylor & Francis Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 History Collection article 2015 ftdatacite https://doi.org/10.6084/m9.figshare.c.1889264 2024-04-02T11:52:31Z In the present study, the cloning and sequence analysis of interleukin‐6 (IL‐6) from the giant panda ( Ailuropoda melanoleuca ) were described. Sequencing revealed that IL‐6 complementary DNA contains a 636 base‐pair open reading frame encoding 211 amino acids. Homology analyses indicated that the identity levels of nucleotide and deduced amino acid sequences of IL‐6 between the giant panda and other species in Carnivora ranged from 90.5% to 85.6% and from 82.9% to 76.5%, respectively. Phylogenetic analyses based on nucleotide and amino acid sequences showed that the harbour seal shared a closer relationship with the giant panda than other carnivores (ursids not included). The functionally important domains and sites that have previously been recognized in other carnivores were all observed in the IL‐6 sequence of the giant panda. These findings suggest that the complementary DNA fragment cloned is indeed the IL‐6 molecule with biological activity and can be used in immunological studies of the giant panda. ... Article in Journal/Newspaper harbour seal DataCite Metadata Store (German National Library of Science and Technology) |
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History Zeng, Bo Yue, Bisong Xu, Liu Peng, Rui Zou, Fangdong Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... |
topic_facet |
History |
description |
In the present study, the cloning and sequence analysis of interleukin‐6 (IL‐6) from the giant panda ( Ailuropoda melanoleuca ) were described. Sequencing revealed that IL‐6 complementary DNA contains a 636 base‐pair open reading frame encoding 211 amino acids. Homology analyses indicated that the identity levels of nucleotide and deduced amino acid sequences of IL‐6 between the giant panda and other species in Carnivora ranged from 90.5% to 85.6% and from 82.9% to 76.5%, respectively. Phylogenetic analyses based on nucleotide and amino acid sequences showed that the harbour seal shared a closer relationship with the giant panda than other carnivores (ursids not included). The functionally important domains and sites that have previously been recognized in other carnivores were all observed in the IL‐6 sequence of the giant panda. These findings suggest that the complementary DNA fragment cloned is indeed the IL‐6 molecule with biological activity and can be used in immunological studies of the giant panda. ... |
format |
Article in Journal/Newspaper |
author |
Zeng, Bo Yue, Bisong Xu, Liu Peng, Rui Zou, Fangdong |
author_facet |
Zeng, Bo Yue, Bisong Xu, Liu Peng, Rui Zou, Fangdong |
author_sort |
Zeng, Bo |
title |
Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... |
title_short |
Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... |
title_full |
Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... |
title_fullStr |
Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... |
title_full_unstemmed |
Molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( Ailuropoda melanoleuca ) ... |
title_sort |
molecular cloning and sequence analysis of the gene encoding interleukin‐6 of the giant panda ( ailuropoda melanoleuca ) ... |
publisher |
Taylor & Francis |
publishDate |
2015 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.1889264 https://tandf.figshare.com/collections/Molecular_cloning_and_sequence_analysis_of_the_gene_encoding_interleukin_6_of_the_giant_panda_i_Ailuropoda_melanoleuca_i_/1889264 |
genre |
harbour seal |
genre_facet |
harbour seal |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.1889264 |
_version_ |
1797584268058165248 |