Northern elephant seal blubber proteome (repeated ACTH challenge)
Juvenile northern elephant seals received once-daily injections of synthetic adrenocorticotropic hormone (ACTH) for four consecutive days. Blubber samples were collected immediately prior to and four hours after the first and fourth ACTH administrations. Proteins were isolated from the inner, metabo...
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ftdatacite:10.6084/m9.figshare.9228869 2023-05-15T16:05:17+02:00 Northern elephant seal blubber proteome (repeated ACTH challenge) Khudyakov, Jane Deyarmin, Jared Hekman, Ryan 2019 https://dx.doi.org/10.6084/m9.figshare.9228869 https://figshare.com/articles/Northern_elephant_seal_blubber_proteome_repeated_ACTH_challenge_/9228869 unknown figshare Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 60802 Animal Cell and Molecular Biology FOS Biological sciences 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Physiology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics Marine Biology dataset Dataset 2019 ftdatacite https://doi.org/10.6084/m9.figshare.9228869 2021-11-05T12:55:41Z Juvenile northern elephant seals received once-daily injections of synthetic adrenocorticotropic hormone (ACTH) for four consecutive days. Blubber samples were collected immediately prior to and four hours after the first and fourth ACTH administrations. Proteins were isolated from the inner, metabolically active half (closest to muscle) of each blubber sample from two study animals for which transcriptomes have been published. Blubber was homogenized in Qiazol and proteins were precipitated from the organic phase after phase extraction with chloroform. Denatured, trypsinized, and alkylated peptides were labeled with iTRAQ 8plex and fractionated off-line using a high pH reverse-phase peptide fractionation kit. Fractions were pooled in pairs and injected onto a C18 LC column. Mass spectrometry analysis was performed on a Thermo Orbitrap Fusion Tribrid mass spectrometer in DDA mode with HCD collision. MS/MS data analysis (SEQUEST search against SwissProt UniProt database) and iTRAQ-labeled peptide quantification at the MS2 level were conducted using Proteome Discoverer v2.2. Dataset Elephant Seal Elephant Seals DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
60802 Animal Cell and Molecular Biology FOS Biological sciences 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Physiology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics Marine Biology |
spellingShingle |
60802 Animal Cell and Molecular Biology FOS Biological sciences 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Physiology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics Marine Biology Khudyakov, Jane Deyarmin, Jared Hekman, Ryan Northern elephant seal blubber proteome (repeated ACTH challenge) |
topic_facet |
60802 Animal Cell and Molecular Biology FOS Biological sciences 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Physiology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics Marine Biology |
description |
Juvenile northern elephant seals received once-daily injections of synthetic adrenocorticotropic hormone (ACTH) for four consecutive days. Blubber samples were collected immediately prior to and four hours after the first and fourth ACTH administrations. Proteins were isolated from the inner, metabolically active half (closest to muscle) of each blubber sample from two study animals for which transcriptomes have been published. Blubber was homogenized in Qiazol and proteins were precipitated from the organic phase after phase extraction with chloroform. Denatured, trypsinized, and alkylated peptides were labeled with iTRAQ 8plex and fractionated off-line using a high pH reverse-phase peptide fractionation kit. Fractions were pooled in pairs and injected onto a C18 LC column. Mass spectrometry analysis was performed on a Thermo Orbitrap Fusion Tribrid mass spectrometer in DDA mode with HCD collision. MS/MS data analysis (SEQUEST search against SwissProt UniProt database) and iTRAQ-labeled peptide quantification at the MS2 level were conducted using Proteome Discoverer v2.2. |
format |
Dataset |
author |
Khudyakov, Jane Deyarmin, Jared Hekman, Ryan |
author_facet |
Khudyakov, Jane Deyarmin, Jared Hekman, Ryan |
author_sort |
Khudyakov, Jane |
title |
Northern elephant seal blubber proteome (repeated ACTH challenge) |
title_short |
Northern elephant seal blubber proteome (repeated ACTH challenge) |
title_full |
Northern elephant seal blubber proteome (repeated ACTH challenge) |
title_fullStr |
Northern elephant seal blubber proteome (repeated ACTH challenge) |
title_full_unstemmed |
Northern elephant seal blubber proteome (repeated ACTH challenge) |
title_sort |
northern elephant seal blubber proteome (repeated acth challenge) |
publisher |
figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.9228869 https://figshare.com/articles/Northern_elephant_seal_blubber_proteome_repeated_ACTH_challenge_/9228869 |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.9228869 |
_version_ |
1766401192416509952 |