MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ...
Additional file 1: Figure S1. PCR analysis of the parental strain GB-4(0) and strain ΔPaEMT1. The primer sets used in PCR amplification to assess PaEMT1 deletion (A). Agarose gel electrophoresis of amplified DNA fragments to confirm gene disruption. The gene fragments were amplified using PaEMT1_inn...
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ftdatacite:10.6084/m9.figshare.8796518 2024-03-31T07:48:34+00:00 MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... Saika, Azusa Koike, Hideaki Yarimizu, Tohru Watanabe, Takashi Kitamoto, Hiroko Morita, Tomotake 2019 https://dx.doi.org/10.6084/m9.figshare.8796518 https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_Deficiency_of_biodegradable_plastic-degrading_enzyme_production_in_a_gene-deletion_mutant_of_phyllosphere_yeast_Pseudozyma_antarctica_defective_in_mannosylerythritol_lipid_biosynthesis/8796518 unknown figshare https://dx.doi.org/10.1186/s13568-019-0825-2 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Chemical Sciences not elsewhere classified Cancer Science Policy Hematology Infectious Diseases FOS Health sciences Text Journal contribution article-journal ScholarlyArticle 2019 ftdatacite https://doi.org/10.6084/m9.figshare.879651810.1186/s13568-019-0825-2 2024-03-04T14:02:26Z Additional file 1: Figure S1. PCR analysis of the parental strain GB-4(0) and strain ΔPaEMT1. The primer sets used in PCR amplification to assess PaEMT1 deletion (A). Agarose gel electrophoresis of amplified DNA fragments to confirm gene disruption. The gene fragments were amplified using PaEMT1_inner_F1 (Primer A) and PaEMT1_inner_R1 (Primer B), or PaEMT1_up_F2 (Primer C) and NATfragment_R1 (Primer D) (B). Figure S2. Schematic diagram of the procedure used to obtain cell-free extract. Figure S3. PaE production by strain ΔPaEMT1 in m-3×FMM medium supplemented with 8 % xylose and various concentrations of TritonX-100. Cell growth (white) and PaE activity (gray) (A) and SDS-PAGE and western blot analyses of culture supernatant (B). CMC, critical micelle concentration; M, marker; P, purified PaE. The amount of each culture supernatant loaded in the gel was 10 μl for CBB staining and western blotting. The results of the cell growth and PaE activity assays are shown as the average of three different experiments. ... Text Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Chemical Sciences not elsewhere classified Cancer Science Policy Hematology Infectious Diseases FOS Health sciences |
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Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Chemical Sciences not elsewhere classified Cancer Science Policy Hematology Infectious Diseases FOS Health sciences Saika, Azusa Koike, Hideaki Yarimizu, Tohru Watanabe, Takashi Kitamoto, Hiroko Morita, Tomotake MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
topic_facet |
Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Chemical Sciences not elsewhere classified Cancer Science Policy Hematology Infectious Diseases FOS Health sciences |
description |
Additional file 1: Figure S1. PCR analysis of the parental strain GB-4(0) and strain ΔPaEMT1. The primer sets used in PCR amplification to assess PaEMT1 deletion (A). Agarose gel electrophoresis of amplified DNA fragments to confirm gene disruption. The gene fragments were amplified using PaEMT1_inner_F1 (Primer A) and PaEMT1_inner_R1 (Primer B), or PaEMT1_up_F2 (Primer C) and NATfragment_R1 (Primer D) (B). Figure S2. Schematic diagram of the procedure used to obtain cell-free extract. Figure S3. PaE production by strain ΔPaEMT1 in m-3×FMM medium supplemented with 8 % xylose and various concentrations of TritonX-100. Cell growth (white) and PaE activity (gray) (A) and SDS-PAGE and western blot analyses of culture supernatant (B). CMC, critical micelle concentration; M, marker; P, purified PaE. The amount of each culture supernatant loaded in the gel was 10 μl for CBB staining and western blotting. The results of the cell growth and PaE activity assays are shown as the average of three different experiments. ... |
format |
Text |
author |
Saika, Azusa Koike, Hideaki Yarimizu, Tohru Watanabe, Takashi Kitamoto, Hiroko Morita, Tomotake |
author_facet |
Saika, Azusa Koike, Hideaki Yarimizu, Tohru Watanabe, Takashi Kitamoto, Hiroko Morita, Tomotake |
author_sort |
Saika, Azusa |
title |
MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
title_short |
MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
title_full |
MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
title_fullStr |
MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
title_full_unstemmed |
MOESM1 of Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
title_sort |
moesm1 of deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ... |
publisher |
figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.8796518 https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_Deficiency_of_biodegradable_plastic-degrading_enzyme_production_in_a_gene-deletion_mutant_of_phyllosphere_yeast_Pseudozyma_antarctica_defective_in_mannosylerythritol_lipid_biosynthesis/8796518 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.1186/s13568-019-0825-2 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.879651810.1186/s13568-019-0825-2 |
_version_ |
1795033035986436096 |