Additional file 1: of The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status

• Main Authors: Crespo, Diego, Bogerd, Jan, Sambroni, Elisabeth, LeGac, Florence, Andersson, Eva, Edvardsen, Rolf, Bergman, Elisabeth, Björnsson, Björn, Taranger, Geir, Schulz, Rüdiger
• Format: Still Image
• Language: unknown
• Published: figshare 2019
• Subjects: Biophysics; Cell Biology; Genetics; FOS Biological sciences; Physiology; 69999 Biological Sciences not elsewhere classified; Developmental Biology; Inorganic Chemistry; FOS Chemical sciences; Atlantic salmon
• Online Access: https://dx.doi.org/10.6084/m9.figshare.8261399.v1; https://springernature.figshare.com/articles/Additional_file_1_of_The_initiation_of_puberty_in_Atlantic_salmon_brings_about_large_changes_in_testicular_gene_expression_that_are_modulated_by_the_energy_status/8261399/1

Figure S1. Morphological evaluation of the maturational status in male salmon. Representative histology (hematoxylin/eosin combined with periodic acid according to Schiff, H/E-PAS; (A-C)) and proliferation activity (immune-detection of phosphorylated histone H3, pH 3; (D-F)) in testes tissue obtained from Atlantic salmon in different stages of maturation. Representative type A undifferentiated (black arrowheads), type A differentiating (black dashed lines), type B spermatogonia (white dashed lines), and “free” Sertoli cells (black arrows) are shown in B-C. In E-F, representative pH 3-positive cells are indicated by red arrowheads (type A undifferentiated spermatogonia), red dashed lines (type B spermatogonia) and red arrows (“free” Sertoli cells), respectively. Scale bar represents 25 (A) or 30 (B-F) μm. (TIF 6792 kb)