TEMPERATURE-INFLUENCED PROTEIN NETWORK DIFFERENCES IN THE PACIFIC OYSTER (CRASSOSTREA GIGAS) DURING LARVAL DEVELOPMENT

• Main Author: Trigg, Shelly A.
• Format: Still Image
• Language: unknown
• Published: figshare 2019
• Subjects: 60802 Animal Cell and Molecular Biology; FOS Biological sciences; 60803 Animal Developmental and Reproductive Biology; 60603 Animal Physiology - Systems; 60102 Bioinformatics; FOS Computer and information sciences; Marine Biology; 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.6084/m9.figshare.7853573.v1; https://figshare.com/articles/TEMPERATURE-INFLUENCED_PROTEIN_NETWORK_DIFFERENCES_IN_THE_PACIFIC_OYSTER_CRASSOSTREA_GIGAS_DURING_LARVAL_DEVELOPMENT/7853573/1

The Pacific oyster has major ecological and economic importance serving as a biofilter and habitat in coastal ecosystems, and contributing over $190M to annual marine aquaculture revenue. However, little is known about the landscape of protein expression during early development, a time when mass mortality is common which can negatively impact industry and ecosystems. To better characterize physiological pathways and associated networks active during oyster development we performed a developmental time series proteomics analysis of larval cultures reared at 23°C and 29°C. These temperatures were selected based on the reports from the aquaculture industry that differential performance is observed in oysters at these temperatures. We found differentially abundant proteins among larval cultures reared at different temperatures. Results from functional protein network analyses provide deeper insight into the mechanisms underlying fundamental developmental processes and mortality events. This proteomics analysis combined with survival and development observations offers greater clarity on environmental conditions that can improve aquaculture production.