Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents
The combination of Deep-eutectic-solvents (DES) with water as “co-solvent” enables a low-viscous reaction medium that keeps its “non-conventional” nature and thus enables synthetic lyophilization reactions (e.g. esterification) catalyzed by hydrolases. Substrates with different polarity may be emplo...
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ftdatacite:10.6084/m9.figshare.7808444 2023-05-15T13:42:25+02:00 Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents Guajardo, Nadia Ahumada, Katherine María, Pablo Domínguez De Schrebler, Rodrigo A. 2019 https://dx.doi.org/10.6084/m9.figshare.7808444 https://tandf.figshare.com/articles/Remarkable_stability_of_i_Candida_antarctica_i_lipase_B_immobilized_i_via_i_cross-linking_aggregates_CLEA_in_deep_eutectic_solvents/7808444 unknown Taylor & Francis https://dx.doi.org/10.1080/10242422.2018.1492567 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Sociology FOS Sociology Inorganic Chemistry 110309 Infectious Diseases FOS Health sciences Text article-journal Journal contribution ScholarlyArticle 2019 ftdatacite https://doi.org/10.6084/m9.figshare.7808444 https://doi.org/10.1080/10242422.2018.1492567 2021-11-05T12:55:41Z The combination of Deep-eutectic-solvents (DES) with water as “co-solvent” enables a low-viscous reaction medium that keeps its “non-conventional” nature and thus enables synthetic lyophilization reactions (e.g. esterification) catalyzed by hydrolases. Substrates with different polarity may be employed. This paper shows how the enzyme immobilization with cross-linking aggregates (CLEA) leads to highly stable and active immobilized catalysts in different DES. As a remarkable case, when choline chloride-glycerol DES is used, CLEA derivatives of Candida antarctica lipase B (CLEA-CALB) are stable for at least 14 days without any loss of activity. The immobilized biocatalysts are applied in non-viscous DES-water blends (8% v/v) to catalyze the esterification of benzoic acid and glycerol to furnish glyceryl monobenzoate (α-MBG) in productivities of ∼35 g α-MBG L −1 d −1 . Compared to other commercial immobilized CALB, the CLEA-CALB derivatives rendered more product (higher conversions by 30%). Moreover, CLEA derivatives were successfully reused for six times without any loss of activity. Given the ease of immobilization (CLEA), their excellent performance in DES and the low viscosity of the DES-water blends, the reported approach may be useful for many synthetic procedures and even for continuous processes with largely optimized outcomes. Text Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Biochemistry Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Sociology FOS Sociology Inorganic Chemistry 110309 Infectious Diseases FOS Health sciences |
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Biochemistry Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Sociology FOS Sociology Inorganic Chemistry 110309 Infectious Diseases FOS Health sciences Guajardo, Nadia Ahumada, Katherine María, Pablo Domínguez De Schrebler, Rodrigo A. Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents |
topic_facet |
Biochemistry Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Sociology FOS Sociology Inorganic Chemistry 110309 Infectious Diseases FOS Health sciences |
description |
The combination of Deep-eutectic-solvents (DES) with water as “co-solvent” enables a low-viscous reaction medium that keeps its “non-conventional” nature and thus enables synthetic lyophilization reactions (e.g. esterification) catalyzed by hydrolases. Substrates with different polarity may be employed. This paper shows how the enzyme immobilization with cross-linking aggregates (CLEA) leads to highly stable and active immobilized catalysts in different DES. As a remarkable case, when choline chloride-glycerol DES is used, CLEA derivatives of Candida antarctica lipase B (CLEA-CALB) are stable for at least 14 days without any loss of activity. The immobilized biocatalysts are applied in non-viscous DES-water blends (8% v/v) to catalyze the esterification of benzoic acid and glycerol to furnish glyceryl monobenzoate (α-MBG) in productivities of ∼35 g α-MBG L −1 d −1 . Compared to other commercial immobilized CALB, the CLEA-CALB derivatives rendered more product (higher conversions by 30%). Moreover, CLEA derivatives were successfully reused for six times without any loss of activity. Given the ease of immobilization (CLEA), their excellent performance in DES and the low viscosity of the DES-water blends, the reported approach may be useful for many synthetic procedures and even for continuous processes with largely optimized outcomes. |
format |
Text |
author |
Guajardo, Nadia Ahumada, Katherine María, Pablo Domínguez De Schrebler, Rodrigo A. |
author_facet |
Guajardo, Nadia Ahumada, Katherine María, Pablo Domínguez De Schrebler, Rodrigo A. |
author_sort |
Guajardo, Nadia |
title |
Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents |
title_short |
Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents |
title_full |
Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents |
title_fullStr |
Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents |
title_full_unstemmed |
Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents |
title_sort |
remarkable stability of candida antarctica lipase b immobilized via cross-linking aggregates (clea) in deep eutectic solvents |
publisher |
Taylor & Francis |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.7808444 https://tandf.figshare.com/articles/Remarkable_stability_of_i_Candida_antarctica_i_lipase_B_immobilized_i_via_i_cross-linking_aggregates_CLEA_in_deep_eutectic_solvents/7808444 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.1080/10242422.2018.1492567 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.7808444 https://doi.org/10.1080/10242422.2018.1492567 |
_version_ |
1766167584402571264 |