CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus
The population diversity and structure of CRISPR–Cas immunity provides key insights into virus–host interactions. Here, we examine two geographically and genetically distinct natural populations of the thermophilic crenarchaeon Sulfolobus islandicus and their interactions with Sulfolobus spindle-sha...
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ftdatacite:10.6084/m9.figshare.7701050 2023-05-15T16:58:59+02:00 CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus Pauly, Matthew D. Bautista, Maria A. Black, Jesse A. Whitaker, Rachel J. 2019 https://dx.doi.org/10.6084/m9.figshare.7701050 https://rs.figshare.com/articles/CRISPR_Spacers_-_List_of_all_spacers_used_for_this_analysis_from_Kamchatka_from_Diversified_local_CRISPR_Cas_immunity_to_viruses_of_i_Sulfolobus_islandicus_i_/7701050 unknown The Royal Society https://dx.doi.org/10.1098/rstb.2018.0093 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Microbiology FOS Biological sciences Evolutionary Biology Text article-journal Journal contribution ScholarlyArticle 2019 ftdatacite https://doi.org/10.6084/m9.figshare.7701050 https://doi.org/10.1098/rstb.2018.0093 2021-11-05T12:55:41Z The population diversity and structure of CRISPR–Cas immunity provides key insights into virus–host interactions. Here, we examine two geographically and genetically distinct natural populations of the thermophilic crenarchaeon Sulfolobus islandicus and their interactions with Sulfolobus spindle-shaped viruses (SSVs) and S. islandicus rod-shaped viruses (SIRVs). We found that both virus families can be targeted with high population distributed immunity, whereby most immune strains target a virus using unique unshared CRISPR spacers. In Kamchatka, Russia, we observed high immunity to chronic SSVs that increases over time. In this context, we found that some SSVs had shortened genomes lacking genes that are highly targeted by the S. islandicus population, indicating a potential mechanism of immune evasion. By contrast, in Yellowstone National Park, we find high inter- and intra-strain immune diversity targeting lytic SIRVs and low immunity to chronic SSVs. In this population, we observed evidence of SIRVs evolving immunity through mutations concentrated in the first five bases of protospacers. These results indicate that diversity and structure of antiviral CRISPR–Cas immunity for a single microbial species can differ by both the population and virus type, and suggest that different virus families use different mechanisms to evade CRISPR–Cas immunity.This article is part of the theme issue ‘The ecology and evolution of prokaryotic CRISPR–Cas adaptive immune systems’. Text Kamchatka DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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topic |
Microbiology FOS Biological sciences Evolutionary Biology |
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Microbiology FOS Biological sciences Evolutionary Biology Pauly, Matthew D. Bautista, Maria A. Black, Jesse A. Whitaker, Rachel J. CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus |
topic_facet |
Microbiology FOS Biological sciences Evolutionary Biology |
description |
The population diversity and structure of CRISPR–Cas immunity provides key insights into virus–host interactions. Here, we examine two geographically and genetically distinct natural populations of the thermophilic crenarchaeon Sulfolobus islandicus and their interactions with Sulfolobus spindle-shaped viruses (SSVs) and S. islandicus rod-shaped viruses (SIRVs). We found that both virus families can be targeted with high population distributed immunity, whereby most immune strains target a virus using unique unshared CRISPR spacers. In Kamchatka, Russia, we observed high immunity to chronic SSVs that increases over time. In this context, we found that some SSVs had shortened genomes lacking genes that are highly targeted by the S. islandicus population, indicating a potential mechanism of immune evasion. By contrast, in Yellowstone National Park, we find high inter- and intra-strain immune diversity targeting lytic SIRVs and low immunity to chronic SSVs. In this population, we observed evidence of SIRVs evolving immunity through mutations concentrated in the first five bases of protospacers. These results indicate that diversity and structure of antiviral CRISPR–Cas immunity for a single microbial species can differ by both the population and virus type, and suggest that different virus families use different mechanisms to evade CRISPR–Cas immunity.This article is part of the theme issue ‘The ecology and evolution of prokaryotic CRISPR–Cas adaptive immune systems’. |
format |
Text |
author |
Pauly, Matthew D. Bautista, Maria A. Black, Jesse A. Whitaker, Rachel J. |
author_facet |
Pauly, Matthew D. Bautista, Maria A. Black, Jesse A. Whitaker, Rachel J. |
author_sort |
Pauly, Matthew D. |
title |
CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus |
title_short |
CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus |
title_full |
CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus |
title_fullStr |
CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus |
title_full_unstemmed |
CRISPR.Spacers - List of all spacers used for this analysis from Kamchatka from Diversified local CRISPR–Cas immunity to viruses of Sulfolobus islandicus |
title_sort |
crispr.spacers - list of all spacers used for this analysis from kamchatka from diversified local crispr–cas immunity to viruses of sulfolobus islandicus |
publisher |
The Royal Society |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.7701050 https://rs.figshare.com/articles/CRISPR_Spacers_-_List_of_all_spacers_used_for_this_analysis_from_Kamchatka_from_Diversified_local_CRISPR_Cas_immunity_to_viruses_of_i_Sulfolobus_islandicus_i_/7701050 |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_relation |
https://dx.doi.org/10.1098/rstb.2018.0093 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.7701050 https://doi.org/10.1098/rstb.2018.0093 |
_version_ |
1766051122562203648 |