Nuclear DNA barcodes for cod identification in mildly-treated and processed food products
Gadoids are a group of fish with historical importance in the fishing industry. The high demand for cod is one of the reasons why cod products are often mislabelled, and numerous observations have been made on the replacement of Atlantic cod ( Gadus morhua ) by cheaper species or its illegal capture...
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ftdatacite:10.6084/m9.figshare.7579919 2023-05-15T15:27:40+02:00 Nuclear DNA barcodes for cod identification in mildly-treated and processed food products Paracchini, Valentina Petrillo, Mauro Lievens, Antoon Dafni-Maria Kagkli Angers-Loustau, Alexandre 2019 https://dx.doi.org/10.6084/m9.figshare.7579919 https://tandf.figshare.com/articles/Nuclear_DNA_barcodes_for_cod_identification_in_mildly-treated_and_processed_food_products/7579919 unknown Taylor & Francis https://dx.doi.org/10.1080/19440049.2018.1556402 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Text article-journal Journal contribution ScholarlyArticle 2019 ftdatacite https://doi.org/10.6084/m9.figshare.7579919 https://doi.org/10.1080/19440049.2018.1556402 2021-11-05T12:55:41Z Gadoids are a group of fish with historical importance in the fishing industry. The high demand for cod is one of the reasons why cod products are often mislabelled, and numerous observations have been made on the replacement of Atlantic cod ( Gadus morhua ) by cheaper species or its illegal capture in contravention of fish quotas. Fish species identification is traditionally based on morphological features, but this may be difficult in case of heat-treated or processed products, or where the species look similar, as in the Gadoid group. DNA-based approaches (using either nuclear or mitochondrial DNA) are most commonly used in this case, due to their high specificity and to the high resilience of the target molecules to food processing techniques. In this article, we identified, using an automated screening approach, novel barcode regions and their associated primers in the nuclear genome, to be used for the efficient identification of Gadoids. The barcode regions were tested on official and commercial samples, raw or mildly treated products, like frozen, or salted, as well as pre-cooked complex mixtures and processed samples, using next-generation sequencing (NGS) technique. The method proposed could complement existing fish identification strategies in establishing an efficient framework to detect and prevent frauds along the food chain. Text atlantic cod Gadus morhua DataCite Metadata Store (German National Library of Science and Technology) |
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Biochemistry Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
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Biochemistry Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Paracchini, Valentina Petrillo, Mauro Lievens, Antoon Dafni-Maria Kagkli Angers-Loustau, Alexandre Nuclear DNA barcodes for cod identification in mildly-treated and processed food products |
topic_facet |
Biochemistry Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
Gadoids are a group of fish with historical importance in the fishing industry. The high demand for cod is one of the reasons why cod products are often mislabelled, and numerous observations have been made on the replacement of Atlantic cod ( Gadus morhua ) by cheaper species or its illegal capture in contravention of fish quotas. Fish species identification is traditionally based on morphological features, but this may be difficult in case of heat-treated or processed products, or where the species look similar, as in the Gadoid group. DNA-based approaches (using either nuclear or mitochondrial DNA) are most commonly used in this case, due to their high specificity and to the high resilience of the target molecules to food processing techniques. In this article, we identified, using an automated screening approach, novel barcode regions and their associated primers in the nuclear genome, to be used for the efficient identification of Gadoids. The barcode regions were tested on official and commercial samples, raw or mildly treated products, like frozen, or salted, as well as pre-cooked complex mixtures and processed samples, using next-generation sequencing (NGS) technique. The method proposed could complement existing fish identification strategies in establishing an efficient framework to detect and prevent frauds along the food chain. |
format |
Text |
author |
Paracchini, Valentina Petrillo, Mauro Lievens, Antoon Dafni-Maria Kagkli Angers-Loustau, Alexandre |
author_facet |
Paracchini, Valentina Petrillo, Mauro Lievens, Antoon Dafni-Maria Kagkli Angers-Loustau, Alexandre |
author_sort |
Paracchini, Valentina |
title |
Nuclear DNA barcodes for cod identification in mildly-treated and processed food products |
title_short |
Nuclear DNA barcodes for cod identification in mildly-treated and processed food products |
title_full |
Nuclear DNA barcodes for cod identification in mildly-treated and processed food products |
title_fullStr |
Nuclear DNA barcodes for cod identification in mildly-treated and processed food products |
title_full_unstemmed |
Nuclear DNA barcodes for cod identification in mildly-treated and processed food products |
title_sort |
nuclear dna barcodes for cod identification in mildly-treated and processed food products |
publisher |
Taylor & Francis |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.7579919 https://tandf.figshare.com/articles/Nuclear_DNA_barcodes_for_cod_identification_in_mildly-treated_and_processed_food_products/7579919 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://dx.doi.org/10.1080/19440049.2018.1556402 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.7579919 https://doi.org/10.1080/19440049.2018.1556402 |
_version_ |
1766358090504994816 |