Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences
Global climate change is rapidly altering coastal marine ecosystems important for food production. A comprehensive understanding of how organisms will respond to these complex environmental changes can come only from observing and studying species within their natural environment. To this end, the e...
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ftdatacite:10.6084/m9.figshare.7450997.v1 2023-05-15T15:58:16+02:00 Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences Yaamini Venkataraman 2018 https://dx.doi.org/10.6084/m9.figshare.7450997.v1 https://figshare.com/articles/Characterization_of_Pacific_oyster_Crassostrea_gigas_proteomic_response_to_natural_environmental_differences/7450997/1 unknown figshare https://dx.doi.org/10.6084/m9.figshare.7450997 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Marine Biology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics FOS Biological sciences Environmental Science 60602 Animal Physiology - Cell dataset Dataset 2018 ftdatacite https://doi.org/10.6084/m9.figshare.7450997.v1 https://doi.org/10.6084/m9.figshare.7450997 2021-11-05T12:55:41Z Global climate change is rapidly altering coastal marine ecosystems important for food production. A comprehensive understanding of how organisms will respond to these complex environmental changes can come only from observing and studying species within their natural environment. To this end, the effects of environmental drivers — pH, dissolved oxygen content, salinity, and temperature — on Pacific oyster (Crassostrea gigas) physiology were evaluated in an outplant experiment. Sibling juvenile oysters were outplanted to eelgrass and unvegetated habitat at five different estuarine sites within the Acidification Nearshore Monitoring Network in Washington State, USA to evaluate how regional environmental drivers influence molecular physiology. Within each site, we also determined if eelgrass presence that buffered pH conditions changed the oysters’ expressed proteome. A novel, two-step, gel-free proteomic approach was used to identify differences in protein abundance in C. gigas ctenidia tissue after a 29 day outplant by 1) identifying proteins in a data independent acquisition survey step and 2) comparing relative quantities of targeted environmental response proteins using selected reaction monitoring. While there was no difference in protein abundance detected between habitats or among sites within Puget Sound, C. gigas outplanted at Willapa Bay had significantly higher abundances of antioxidant enzymes and molecular chaperones. Environmental factors at Willapa Bay, such as higher average temperature, may have driven this protein abundance pattern. These findings generate a suite of new hypotheses for lab and field experiments to compare the effects of regional conditions on physiological responses of marine invertebrates. Dataset Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
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language |
unknown |
topic |
Marine Biology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics FOS Biological sciences Environmental Science 60602 Animal Physiology - Cell |
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Marine Biology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics FOS Biological sciences Environmental Science 60602 Animal Physiology - Cell Yaamini Venkataraman Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences |
topic_facet |
Marine Biology 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics FOS Biological sciences Environmental Science 60602 Animal Physiology - Cell |
description |
Global climate change is rapidly altering coastal marine ecosystems important for food production. A comprehensive understanding of how organisms will respond to these complex environmental changes can come only from observing and studying species within their natural environment. To this end, the effects of environmental drivers — pH, dissolved oxygen content, salinity, and temperature — on Pacific oyster (Crassostrea gigas) physiology were evaluated in an outplant experiment. Sibling juvenile oysters were outplanted to eelgrass and unvegetated habitat at five different estuarine sites within the Acidification Nearshore Monitoring Network in Washington State, USA to evaluate how regional environmental drivers influence molecular physiology. Within each site, we also determined if eelgrass presence that buffered pH conditions changed the oysters’ expressed proteome. A novel, two-step, gel-free proteomic approach was used to identify differences in protein abundance in C. gigas ctenidia tissue after a 29 day outplant by 1) identifying proteins in a data independent acquisition survey step and 2) comparing relative quantities of targeted environmental response proteins using selected reaction monitoring. While there was no difference in protein abundance detected between habitats or among sites within Puget Sound, C. gigas outplanted at Willapa Bay had significantly higher abundances of antioxidant enzymes and molecular chaperones. Environmental factors at Willapa Bay, such as higher average temperature, may have driven this protein abundance pattern. These findings generate a suite of new hypotheses for lab and field experiments to compare the effects of regional conditions on physiological responses of marine invertebrates. |
format |
Dataset |
author |
Yaamini Venkataraman |
author_facet |
Yaamini Venkataraman |
author_sort |
Yaamini Venkataraman |
title |
Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences |
title_short |
Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences |
title_full |
Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences |
title_fullStr |
Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences |
title_full_unstemmed |
Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences |
title_sort |
characterization of pacific oyster (crassostrea gigas) proteomic response to natural environmental differences |
publisher |
figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.7450997.v1 https://figshare.com/articles/Characterization_of_Pacific_oyster_Crassostrea_gigas_proteomic_response_to_natural_environmental_differences/7450997/1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://dx.doi.org/10.6084/m9.figshare.7450997 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.7450997.v1 https://doi.org/10.6084/m9.figshare.7450997 |
_version_ |
1766394001766744064 |