Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon
Stress experienced during early life may have lasting effects on the immune system, with impacts on health and disease dependent on the nature and duration of the stressor. The epigenome is especially sensitive to environmental stimuli during early life and represents a potential mechanism through w...
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ftdatacite:10.6084/m9.figshare.7448834.v1 2023-05-15T15:31:54+02:00 Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon Tamsyn M. Uren Webster Deiene Rodriguez-Barreto Martin, Samuel A.M. Oosterhout, Cock Van Orozco-TerWengel, Pablo Cable, Joanne Hamilton, Alastair Leaniz, Carlos Garcia De Consuegra, Sofia 2018 https://dx.doi.org/10.6084/m9.figshare.7448834.v1 https://tandf.figshare.com/articles/Contrasting_effects_of_acute_and_chronic_stress_on_the_transcriptome_epigenome_and_immune_response_of_Atlantic_salmon/7448834/1 unknown Taylor & Francis https://dx.doi.org/10.1080/15592294.2018.1554520 https://dx.doi.org/10.6084/m9.figshare.7448834 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Science Policy 111714 Mental Health FOS Health sciences Other CreativeWork Online resource article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.7448834.v1 https://doi.org/10.1080/15592294.2018.1554520 https://doi.org/10.6084/m9.figshare.7448834 2021-11-05T12:55:41Z Stress experienced during early life may have lasting effects on the immune system, with impacts on health and disease dependent on the nature and duration of the stressor. The epigenome is especially sensitive to environmental stimuli during early life and represents a potential mechanism through which stress may cause long-lasting health effects. However, the extent to which the epigenome responds differently to chronic vs acute stressors is unclear, especially for non-mammalian species. We examined the effects of acute stress (cold-shock during embryogenesis) and chronic stress (absence of tank enrichment during larval-stage) on global gene expression (using RNA-seq) and DNA methylation (using RRBS) in the gills of Atlantic salmon ( Salmo salar ) four months after hatching. Chronic stress induced pronounced transcriptional differences, while acute stress caused few lasting transcriptional effects. However, both acute and chronic stress caused lasting and contrasting changes in the methylome. Crucially, we found that acute stress enhanced transcriptional immune response to a pathogenic challenge (bacterial lipopolysaccharide, LPS), while chronic stress suppressed it. We identified stress-induced changes in promoter and gene-body methylation that were associated with altered expression for a small proportion of immune-related genes, and evidence of wider epigenetic regulation within signalling pathways involved in immune response. Our results suggest that stress can affect immuno-competence through epigenetic mechanisms, and highlight the markedly different effects of chronic larval and acute embryonic stress. This knowledge could be used to harness the stimulatory effects of acute stress on immunity, paving the way for improved stress and disease management through epigenetic conditioning. Article in Journal/Newspaper Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Science Policy 111714 Mental Health FOS Health sciences |
spellingShingle |
Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Science Policy 111714 Mental Health FOS Health sciences Tamsyn M. Uren Webster Deiene Rodriguez-Barreto Martin, Samuel A.M. Oosterhout, Cock Van Orozco-TerWengel, Pablo Cable, Joanne Hamilton, Alastair Leaniz, Carlos Garcia De Consuegra, Sofia Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon |
topic_facet |
Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Science Policy 111714 Mental Health FOS Health sciences |
description |
Stress experienced during early life may have lasting effects on the immune system, with impacts on health and disease dependent on the nature and duration of the stressor. The epigenome is especially sensitive to environmental stimuli during early life and represents a potential mechanism through which stress may cause long-lasting health effects. However, the extent to which the epigenome responds differently to chronic vs acute stressors is unclear, especially for non-mammalian species. We examined the effects of acute stress (cold-shock during embryogenesis) and chronic stress (absence of tank enrichment during larval-stage) on global gene expression (using RNA-seq) and DNA methylation (using RRBS) in the gills of Atlantic salmon ( Salmo salar ) four months after hatching. Chronic stress induced pronounced transcriptional differences, while acute stress caused few lasting transcriptional effects. However, both acute and chronic stress caused lasting and contrasting changes in the methylome. Crucially, we found that acute stress enhanced transcriptional immune response to a pathogenic challenge (bacterial lipopolysaccharide, LPS), while chronic stress suppressed it. We identified stress-induced changes in promoter and gene-body methylation that were associated with altered expression for a small proportion of immune-related genes, and evidence of wider epigenetic regulation within signalling pathways involved in immune response. Our results suggest that stress can affect immuno-competence through epigenetic mechanisms, and highlight the markedly different effects of chronic larval and acute embryonic stress. This knowledge could be used to harness the stimulatory effects of acute stress on immunity, paving the way for improved stress and disease management through epigenetic conditioning. |
format |
Article in Journal/Newspaper |
author |
Tamsyn M. Uren Webster Deiene Rodriguez-Barreto Martin, Samuel A.M. Oosterhout, Cock Van Orozco-TerWengel, Pablo Cable, Joanne Hamilton, Alastair Leaniz, Carlos Garcia De Consuegra, Sofia |
author_facet |
Tamsyn M. Uren Webster Deiene Rodriguez-Barreto Martin, Samuel A.M. Oosterhout, Cock Van Orozco-TerWengel, Pablo Cable, Joanne Hamilton, Alastair Leaniz, Carlos Garcia De Consuegra, Sofia |
author_sort |
Tamsyn M. Uren Webster |
title |
Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon |
title_short |
Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon |
title_full |
Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon |
title_fullStr |
Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon |
title_full_unstemmed |
Contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of Atlantic salmon |
title_sort |
contrasting effects of acute and chronic stress on the transcriptome, epigenome, and immune response of atlantic salmon |
publisher |
Taylor & Francis |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.7448834.v1 https://tandf.figshare.com/articles/Contrasting_effects_of_acute_and_chronic_stress_on_the_transcriptome_epigenome_and_immune_response_of_Atlantic_salmon/7448834/1 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1080/15592294.2018.1554520 https://dx.doi.org/10.6084/m9.figshare.7448834 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.7448834.v1 https://doi.org/10.1080/15592294.2018.1554520 https://doi.org/10.6084/m9.figshare.7448834 |
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1766362397544546304 |