Halibut mitochondrial genomes contain extensive heteroplasmic tandem repeat arrays involved in DNA recombination-2

• Main Authors: Mjelle, Kenneth A, Karlsen, Bård O, Jørgensen, Tor E, Moum, Truls, Johansen, Steinar D
• Format: Still Image
• Language: unknown
• Published: figshare 2011
• Subjects: Molecular Biology; Greenland; Pacific
• Online Access: https://dx.doi.org/10.6084/m9.figshare.73020; https://figshare.com/articles/figure/Halibut_mitochondrial_genomes_contain_extensive_heteroplasmic_tandem_repeat_arrays_involved_in_DNA_recombination-2/73020

Copyright information: Taken from "Halibut mitochondrial genomes contain extensive heteroplasmic tandem repeat arrays involved in DNA recombination"http://www.biomedcentral.com/1471-2164/9/10BMC Genomics 2008;9():10-10.Published online 11 Jan 2008PMCID:PMC2248175.FP) and a reverse primer (RP). Here, primer sets L16293/H17347 and L16376/H17369 were used on Atlantic/Pacific halibuts and Greenland halibut, respectively. () Separation of amplified products in a 2.5% agarose gel. M, the size marker 1 Kb Plus DNA Ladder from Invitrogen (right). Number of repeat motifs is indicated (left). The four smallest amplified fragments from Hh-2 (marked a, b, c, and d) were eluted from the gel and further sequence analysed. () Summary of sequence analyses of fragment from Hh-2. Note that fragment contains three repeats of the 61-bp motif (I*, II, II), fragment contains two repeat motifs (II, II), fragment contains a single motif (II), and fragment lacks a complete motif. Motif I* is not present in the plasmid cloned array of Hh-2 (Figure 2) and may represent site heteroplasmy at array motif position 33.