Modelling the long-term feed-to-fillet transfer of leuco crystal violet and leuco malachite green in Atlantic salmon ( Salmo salar )

• Main Authors: Berntssen, Marc HG, Hannisdal, Rita, Buttle, Louise, Hoogenveen, Rudolf, Mengelers, Marcel, Bokkers, Bas GH, Zeilmaker, Marco J
• Format: Text
• Language: unknown
• Published: Taylor & Francis 2018
• Subjects: Cell Biology; Biotechnology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; Ecology; FOS Biological sciences; 69999 Biological Sciences not elsewhere classified; Inorganic Chemistry; FOS Chemical sciences; Science Policy; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.6809924.v1; https://tandf.figshare.com/articles/Modelling_the_long-term_feed-to-fillet_transfer_of_leuco_crystal_violet_and_leuco_malachite_green_in_Atlantic_salmon_i_Salmo_salar_i_/6809924/1

Leuco crystal violet (LCV) and leuco malachite green (LMG) are the main metabolites of two dyes that are forbidden for use in food production, but can be present at low background concentration in novel Atlantic salmon feed ingredients such as processed animal proteins (ABP). In this study, the potential transfer of dietary LCV or LMG to the fillet of farmed Atlantic salmon was investigated. The uptake and elimination rate kinetics were determined in seawater-adapted Atlantic salmon (initial weight 587 ± 148 g) fed two levels of either LCV or LMG enriched diets (~500 and 4000 µg kg −1 , respectively) for 40 days, followed by a 90 days depuration period with feeding on control diets (< 0.15 μg kg −1 LCV and LMG). A three-compartmental model was developed, based on a fillet fat, fillet muscle, and a central body compartment comprising all other tissues. Model calibrations showed a good fit with measured values during overall uptake and elimination period, however, the model poorly predicted the short-term (days) peak measured values at the end of the exposure period. The model was used to simulate the long term (>16 months) LCV and LMG feed-to fillet transfer in Atlantic salmon under realistic farming conditions such as the seasonal fluctuations in feed intake, growth, and fillet fat deposition. The model predictions gave highest expected LCV and LMG fillet concentrations of approximately 0.12 and 0.45 μg kg −1 , depending on the dietary levels of ABP and background level of LCV and LMG contamination. These levels are under the reference point for action (RPA) of 2 µg kg −1 for the sum of MG and LMG that EFSA assessed as adequate to protect public health. However, for LCV the predicted highest levels exceed the analytical decision limit (CCα) of 0.15 µg kg −1 for the method used in this paper.