Elephant seal outer blubber proteome
Proteins were extracted from the outer (closest to skin) blubber layer of 4 northern elephant seals using either a standard detergent lysis buffer (1% sodium deoxycholate, 8 M urea, 5 mM DTT in 50 mM ammonium bicarbonate; SDC method) or a phenol and guanidine thiocyanate reagent (Qiazol; QIA method)...
Main Author: | |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
figshare
2018
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.6399881 https://figshare.com/articles/Elephant_seal_outer_blubber_protein_sequences/6399881 |
id |
ftdatacite:10.6084/m9.figshare.6399881 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.6399881 2023-05-15T16:05:16+02:00 Elephant seal outer blubber proteome Khudyakov, Jane 2018 https://dx.doi.org/10.6084/m9.figshare.6399881 https://figshare.com/articles/Elephant_seal_outer_blubber_protein_sequences/6399881 unknown figshare Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 60602 Animal Physiology - Cell FOS Biological sciences Biological Techniques 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics 60604 Comparative Physiology dataset Dataset 2018 ftdatacite https://doi.org/10.6084/m9.figshare.6399881 2021-11-05T12:55:41Z Proteins were extracted from the outer (closest to skin) blubber layer of 4 northern elephant seals using either a standard detergent lysis buffer (1% sodium deoxycholate, 8 M urea, 5 mM DTT in 50 mM ammonium bicarbonate; SDC method) or a phenol and guanidine thiocyanate reagent (Qiazol; QIA method). Tandem mass spectrometry analysis was performed using an Orbitrap Fusion Tribrid instrument in DDA mode. Precursor ions were fragmented using stepped collision HCD. MS/MS data was analyzed using Proteome Discoverer v2.2 and PEAKS v8.5. This dataset contains lists of proteins identified by SEQUEST (in Proteome Discoverer) and PEAKS DB searches against the UniProt SwissProt (2/13/18) database (< 1% FDR) and de novo peptide sequences obtained by PEAKS (fasta files) for proteins extracted using QIA and SDC methods. Dataset Elephant Seal Elephant Seals DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
60602 Animal Physiology - Cell FOS Biological sciences Biological Techniques 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics 60604 Comparative Physiology |
spellingShingle |
60602 Animal Physiology - Cell FOS Biological sciences Biological Techniques 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics 60604 Comparative Physiology Khudyakov, Jane Elephant seal outer blubber proteome |
topic_facet |
60602 Animal Physiology - Cell FOS Biological sciences Biological Techniques 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics 60604 Comparative Physiology |
description |
Proteins were extracted from the outer (closest to skin) blubber layer of 4 northern elephant seals using either a standard detergent lysis buffer (1% sodium deoxycholate, 8 M urea, 5 mM DTT in 50 mM ammonium bicarbonate; SDC method) or a phenol and guanidine thiocyanate reagent (Qiazol; QIA method). Tandem mass spectrometry analysis was performed using an Orbitrap Fusion Tribrid instrument in DDA mode. Precursor ions were fragmented using stepped collision HCD. MS/MS data was analyzed using Proteome Discoverer v2.2 and PEAKS v8.5. This dataset contains lists of proteins identified by SEQUEST (in Proteome Discoverer) and PEAKS DB searches against the UniProt SwissProt (2/13/18) database (< 1% FDR) and de novo peptide sequences obtained by PEAKS (fasta files) for proteins extracted using QIA and SDC methods. |
format |
Dataset |
author |
Khudyakov, Jane |
author_facet |
Khudyakov, Jane |
author_sort |
Khudyakov, Jane |
title |
Elephant seal outer blubber proteome |
title_short |
Elephant seal outer blubber proteome |
title_full |
Elephant seal outer blubber proteome |
title_fullStr |
Elephant seal outer blubber proteome |
title_full_unstemmed |
Elephant seal outer blubber proteome |
title_sort |
elephant seal outer blubber proteome |
publisher |
figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.6399881 https://figshare.com/articles/Elephant_seal_outer_blubber_protein_sequences/6399881 |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.6399881 |
_version_ |
1766401175917166592 |