Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil
ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being releva...
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2018
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ftdatacite:10.6084/m9.figshare.6208085 2023-05-15T17:47:21+02:00 Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas 2018 https://dx.doi.org/10.6084/m9.figshare.6208085 https://scielo.figshare.com/articles/Investigation_of_Enteric_Viruses_in_the_Feces_of_Neotropical_Migratory_Birds_Captured_on_the_Coast_of_the_State_of_Par_Brazil/6208085 unknown SciELO journals https://dx.doi.org/10.1590/1806-9061-2017-0589 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 60801 Animal Behaviour FOS Biological sciences 100199 Agricultural Biotechnology not elsewhere classified FOS Agricultural biotechnology dataset Dataset 2018 ftdatacite https://doi.org/10.6084/m9.figshare.6208085 https://doi.org/10.1590/1806-9061-2017-0589 2021-11-05T12:55:41Z ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. Dataset Numenius phaeopus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
60801 Animal Behaviour FOS Biological sciences 100199 Agricultural Biotechnology not elsewhere classified FOS Agricultural biotechnology |
spellingShingle |
60801 Animal Behaviour FOS Biological sciences 100199 Agricultural Biotechnology not elsewhere classified FOS Agricultural biotechnology AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
topic_facet |
60801 Animal Behaviour FOS Biological sciences 100199 Agricultural Biotechnology not elsewhere classified FOS Agricultural biotechnology |
description |
ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. |
format |
Dataset |
author |
AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas |
author_facet |
AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas |
author_sort |
AN Guerreiro |
title |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_short |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_full |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_fullStr |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_full_unstemmed |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_sort |
investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of pará, brazil |
publisher |
SciELO journals |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.6208085 https://scielo.figshare.com/articles/Investigation_of_Enteric_Viruses_in_the_Feces_of_Neotropical_Migratory_Birds_Captured_on_the_Coast_of_the_State_of_Par_Brazil/6208085 |
genre |
Numenius phaeopus |
genre_facet |
Numenius phaeopus |
op_relation |
https://dx.doi.org/10.1590/1806-9061-2017-0589 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.6208085 https://doi.org/10.1590/1806-9061-2017-0589 |
_version_ |
1766151738130169856 |