In silico analysis and characterization of fresh water fish ATPases and homology modelling

• Main Authors: Rumpi Ghosh, AD Upadhayay, AK Roy
• Format: Text
• Language: unknown
• Published: figshare 2017
• Subjects: 60102 Bioinformatics; FOS Computer and information sciences; 110106 Medical Biochemistry Proteins and Peptides incl. Medical Proteomics; FOS Clinical medicine; 60109 Proteomics and Intermolecular Interactions excl. Medical Proteomics; FOS Biological sciences; Coregonus autumnalis
• Online Access: https://dx.doi.org/10.6084/m9.figshare.5631910; https://figshare.com/articles/journal_contribution/In_silico_analysis_and_characterization_of_fresh_water_fish_ATPases_and_homology_modelling/5631910

ATPases is known to be a crucial in many biological activities of organisms. In this study, physicochemical properties and modeling of ATPases protein of fish was analysed using In silico approach. ATPases a protein selected from fish species, including Gold fish ( Carassius auratus auratus ), Zebra fish ( Hypancistrus zebra ), White fishes ( Coregonus autumnalis ), Grass carp ( Ctenopharyngodon idella ) and Anabas testudineus (Koi) were used in this study. Physicochemical characteristics showed with molecular weight (25045.58-25148.57Da), theoretical isoelectric point (9.30-9.97), extinction coefficient(26470-34950), aliphatic index(147.31-150.35), instability index(32.84-42.67), total number of negatively charged residues and positively charged residues (5/7-6/8), and grand average of hydropathicity (1.014-1.151) were computed. All proteins were classified as transmembrane proteins. In secondary structure prediction, all proteins were composed of random coils as predominant, followed by extended strands, alpha helix and beta turn. Three dimensional structure of protein were predicted and verified as good structures. All model structures were evaluated being accepted and reliable based on structural evaluation and stereo chemical analysis.