Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes
Contaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of...
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ftdatacite:10.6084/m9.figshare.5368744 2023-05-15T14:41:22+02:00 Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes Petersen, Karina Hultman, Maria T. Bytingsvik, Jenny Harju, Mikael Evenset, Anita Tollefsen, Knut Erik 2017 https://dx.doi.org/10.6084/m9.figshare.5368744 https://tandf.figshare.com/articles/journal_contribution/Characterizing_cytotoxic_and_estrogenic_activity_of_Arctic_char_tissue_extracts_in_primary_Arctic_char_hepatocytes/5368744 unknown Taylor & Francis https://dx.doi.org/10.1080/15287394.2017.1357277 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Text article-journal Journal contribution ScholarlyArticle 2017 ftdatacite https://doi.org/10.6084/m9.figshare.5368744 https://doi.org/10.1080/15287394.2017.1357277 2021-11-05T12:55:41Z Contaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of contamination were found in freshwater systems such as Lake Ellasjøen at Bjørnøya (Bear Island, Norway). Higher concentrations of organic halogenated compounds (OHC), and higher levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic char ( Salvelinus alpinus ) from this lake compared to fish from other lakes on Bjørnøya. Although several of the measured contaminants are potential endocrine disrupters, few studies have investigated potential endocrine disruptive effects of the contaminant cocktail in this fish population. The aim of this study was to compare acutely toxic and estrogenic potency of the cocktail of pollutants as evidenced by cytotoxic and/or estrogenic effects in vitro using extracts of Arctic char livers from contaminated Lake Ellasjøen with those from less contaminated Lake Laksvatn at Bjørnøya. This was performed by in situ sampling and contaminant extraction from liver tissue, followed by chemical analysis and in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels were highest in extracts from Ellasjøen fish. The F2 and F3 extracts from Lake Laksvatn and Lake Ellasjøen fish reduced in vitro cell viability at a concentration ratio of 0.03–1 relative to tissue concentration in Arctic char. Only the F3 liver extract from Ellasjøen fish increased in vitro vitellogenin protein expression. Although compounds such as estrogenic OH-PCBs were quantified in Ellasjøen F3 extracts, it remains to be determined which compounds were inducing estrogenic effects. Text Arctic Bear Island Bjørnøya Bjørnøya Salvelinus alpinus DataCite Metadata Store (German National Library of Science and Technology) Arctic Bear Island ENVELOPE(-67.250,-67.250,-68.151,-68.151) Bjørnøya ENVELOPE(-67.250,-67.250,-68.151,-68.151) Ellasjøen ENVELOPE(19.031,19.031,74.388,74.388) Laksvatn ENVELOPE(19.360,19.360,69.361,69.361) Norway |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
spellingShingle |
Biochemistry Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Petersen, Karina Hultman, Maria T. Bytingsvik, Jenny Harju, Mikael Evenset, Anita Tollefsen, Knut Erik Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes |
topic_facet |
Biochemistry Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
description |
Contaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of contamination were found in freshwater systems such as Lake Ellasjøen at Bjørnøya (Bear Island, Norway). Higher concentrations of organic halogenated compounds (OHC), and higher levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic char ( Salvelinus alpinus ) from this lake compared to fish from other lakes on Bjørnøya. Although several of the measured contaminants are potential endocrine disrupters, few studies have investigated potential endocrine disruptive effects of the contaminant cocktail in this fish population. The aim of this study was to compare acutely toxic and estrogenic potency of the cocktail of pollutants as evidenced by cytotoxic and/or estrogenic effects in vitro using extracts of Arctic char livers from contaminated Lake Ellasjøen with those from less contaminated Lake Laksvatn at Bjørnøya. This was performed by in situ sampling and contaminant extraction from liver tissue, followed by chemical analysis and in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels were highest in extracts from Ellasjøen fish. The F2 and F3 extracts from Lake Laksvatn and Lake Ellasjøen fish reduced in vitro cell viability at a concentration ratio of 0.03–1 relative to tissue concentration in Arctic char. Only the F3 liver extract from Ellasjøen fish increased in vitro vitellogenin protein expression. Although compounds such as estrogenic OH-PCBs were quantified in Ellasjøen F3 extracts, it remains to be determined which compounds were inducing estrogenic effects. |
format |
Text |
author |
Petersen, Karina Hultman, Maria T. Bytingsvik, Jenny Harju, Mikael Evenset, Anita Tollefsen, Knut Erik |
author_facet |
Petersen, Karina Hultman, Maria T. Bytingsvik, Jenny Harju, Mikael Evenset, Anita Tollefsen, Knut Erik |
author_sort |
Petersen, Karina |
title |
Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes |
title_short |
Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes |
title_full |
Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes |
title_fullStr |
Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes |
title_full_unstemmed |
Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes |
title_sort |
characterizing cytotoxic and estrogenic activity of arctic char tissue extracts in primary arctic char hepatocytes |
publisher |
Taylor & Francis |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.5368744 https://tandf.figshare.com/articles/journal_contribution/Characterizing_cytotoxic_and_estrogenic_activity_of_Arctic_char_tissue_extracts_in_primary_Arctic_char_hepatocytes/5368744 |
long_lat |
ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(19.031,19.031,74.388,74.388) ENVELOPE(19.360,19.360,69.361,69.361) |
geographic |
Arctic Bear Island Bjørnøya Ellasjøen Laksvatn Norway |
geographic_facet |
Arctic Bear Island Bjørnøya Ellasjøen Laksvatn Norway |
genre |
Arctic Bear Island Bjørnøya Bjørnøya Salvelinus alpinus |
genre_facet |
Arctic Bear Island Bjørnøya Bjørnøya Salvelinus alpinus |
op_relation |
https://dx.doi.org/10.1080/15287394.2017.1357277 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.5368744 https://doi.org/10.1080/15287394.2017.1357277 |
_version_ |
1766313153504739328 |