Enzymatic synthesis and anti-oxidative activities of plant oil-based ascorbyl esters in 2-methyltetrahydrofuran-containing mixtures

• Main Authors: Ying-Dan Hu, Zong, Min-Hua, Li, Ning
• Format: Text
• Language: unknown
• Published: Taylor & Francis 2016
• Subjects: Biochemistry; Microbiology; FOS Biological sciences; Genetics; Biotechnology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; 39999 Chemical Sciences not elsewhere classified; FOS Chemical sciences; Ecology; 69999 Biological Sciences not elsewhere classified; Plant Biology; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.6084/m9.figshare.4239434.v1; https://tandf.figshare.com/articles/journal_contribution/Enzymatic_synthesis_and_anti-oxidative_activities_of_plant_oil-based_ascorbyl_esters_in_2-methyltetrahydrofuran-containing_mixtures/4239434/1

Ascorbyl fatty acid esters are commercially interesting fat-soluble antioxidants. In this work, enzymatic synthesis of ascorbyl esters from less expensive and readily available plant oils, and their anti-oxidative activities are described. Among the immobilized lipases tested, Candida antarctica lipase B was the best for the synthesis of plant oil-based ascorbyl esters. The enzyme showed much better catalytic performances in the binary mixtures of biomass-based 2-methyltetrahydrofuran (MeTHF) and t -butanol than the previously preferred t -butanol. The conversions of 70–73% were obtained under the optimal reaction conditions after 24 h, with the unsaturated fatty acid esters (oleate and linoleate, 80–90%) as the major products. The immobilized lipase kept the relative activity of 80% after reuse for 6 batches in MeTHF-containing system. Besides, anti-oxidative activities of plant oil-based ascorbyl esters and ascorbic acid were comparable, which could remove α,α-diphenyl-β-picrylhydrazyl (DPPH) free radical of >87%.