Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection

• Main Author: Rosales, Stephanie
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2016
• Subjects: Marine Biology; 60804 Animal Immunology; FOS Biological sciences; 60805 Animal Neurobiology; 60102 Bioinformatics; FOS Computer and information sciences; 60104 Cell Metabolism; 60604 Comparative Physiology; Computational Biology; Zoology; 60506 Virology; 60809 Vertebrate Biology; Pathology; Lanes; Weddell; harbor seal; Phoca vitulina; Weddell Seal
• Online Access: https://dx.doi.org/10.6084/m9.figshare.3581712.v1; https://figshare.com/articles/software/Brain_transcriptomes_of_harbor_seals_demonstrate_gene_expression_patterns_of_animals_undergoing_a_metabolic_disease_and_a_viral_infection/3581712/1

Harbor seal ( Phoca vitulina) transcriptome libraries were prepared by using 11 harbor seal brain tissue samples. A disposable pestle was used to homogenize ~0.5ng of frozen brain in Trizol (Life Technologies, CA). The homogenate was centrifuged for 10min at 12,000 x g at 4°C, and the supernatant tr...