Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection
Harbor seal ( Phoca vitulina) transcriptome libraries were prepared by using 11 harbor seal brain tissue samples. A disposable pestle was used to homogenize ~0.5ng of frozen brain in Trizol (Life Technologies, CA). The homogenate was centrifuged for 10min at 12,000 x g at 4°C, and the supernatant tr...
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ftdatacite:10.6084/m9.figshare.3581712.v1 2023-05-15T16:33:04+02:00 Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection Rosales, Stephanie 2016 https://dx.doi.org/10.6084/m9.figshare.3581712.v1 https://figshare.com/articles/software/Brain_transcriptomes_of_harbor_seals_demonstrate_gene_expression_patterns_of_animals_undergoing_a_metabolic_disease_and_a_viral_infection/3581712/1 unknown figshare https://dx.doi.org/10.6084/m9.figshare.3581712 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Marine Biology 60804 Animal Immunology FOS Biological sciences 60805 Animal Neurobiology 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Computational Biology Zoology 60506 Virology 60809 Vertebrate Biology Pathology Software SoftwareSourceCode article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.3581712.v1 https://doi.org/10.6084/m9.figshare.3581712 2021-11-05T12:55:41Z Harbor seal ( Phoca vitulina) transcriptome libraries were prepared by using 11 harbor seal brain tissue samples. A disposable pestle was used to homogenize ~0.5ng of frozen brain in Trizol (Life Technologies, CA). The homogenate was centrifuged for 10min at 12,000 x g at 4°C, and the supernatant transferred to a clean tube. For every 1 mL of Trizol, 0.2mL of chloroform was added to the supernatant, vortexed briefly, and centrifuged at 10,000 x g for 18 minutes at 4°C. The aqueous layer was then transferred to a clean tube and equal volumes of 100% ethanol were added to samples, and loaded onto an RNeasy column for extraction as recommended by the manufacturer (Qiagen, CA). To remove DNA, samples were exposed to 2U of Turbo DNase (Life Technologies, CA) for 9 hours at 37°C. Harbor seal rRNA was removed using the Ribo-Zero Kit Gold (Human-Mouse-Rat) from Epicentre (WI, USA) following the manufacturer’s directions. High quality RNA was converted to cDNA using superscript II Reverse Transcriptase (Life Technologies, CA). Libraries were prepared for each of the 11 samples using the TruSeq paired end cluster kit v.3 from Illumina (San Diego, CA). Libraries were sequenced on two lanes of the Illumina Hi-Seq 2000 platform.Harbor seal brain transcriptome assembly was conducted using a combination of transcriptome guided and de novo methods. Eleven quality assured sequence libraries were combined and aligned to the hypothetical Weddell seal, Leptonychotes weddellii (NCBI accession: PRJNA232772) transcriptome, using the program Bowtie2-2.2.3 (21). Aligned sequences were used to build a de novo harbor seal transcriptome using Trinity 2.0.6 with parameters --single, and –full-cleanup. The longest representative transcript for each component or subcomponent in the transcriptome assembly was selected using trinity_reps.pl (https://goo.gl/EGq7I6 ). The National Marine Fisheries (NMFS) authorized the collection of tissue samples from stranded marine mammals and satisfies The Marine Mammal Protection Act (MMPA) regulation 50 CR 216.22 and 216.37. Article in Journal/Newspaper harbor seal Phoca vitulina Weddell Seal DataCite Metadata Store (German National Library of Science and Technology) Lanes ENVELOPE(18.933,18.933,69.617,69.617) Weddell |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Marine Biology 60804 Animal Immunology FOS Biological sciences 60805 Animal Neurobiology 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Computational Biology Zoology 60506 Virology 60809 Vertebrate Biology Pathology |
spellingShingle |
Marine Biology 60804 Animal Immunology FOS Biological sciences 60805 Animal Neurobiology 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Computational Biology Zoology 60506 Virology 60809 Vertebrate Biology Pathology Rosales, Stephanie Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
topic_facet |
Marine Biology 60804 Animal Immunology FOS Biological sciences 60805 Animal Neurobiology 60102 Bioinformatics FOS Computer and information sciences 60104 Cell Metabolism 60604 Comparative Physiology Computational Biology Zoology 60506 Virology 60809 Vertebrate Biology Pathology |
description |
Harbor seal ( Phoca vitulina) transcriptome libraries were prepared by using 11 harbor seal brain tissue samples. A disposable pestle was used to homogenize ~0.5ng of frozen brain in Trizol (Life Technologies, CA). The homogenate was centrifuged for 10min at 12,000 x g at 4°C, and the supernatant transferred to a clean tube. For every 1 mL of Trizol, 0.2mL of chloroform was added to the supernatant, vortexed briefly, and centrifuged at 10,000 x g for 18 minutes at 4°C. The aqueous layer was then transferred to a clean tube and equal volumes of 100% ethanol were added to samples, and loaded onto an RNeasy column for extraction as recommended by the manufacturer (Qiagen, CA). To remove DNA, samples were exposed to 2U of Turbo DNase (Life Technologies, CA) for 9 hours at 37°C. Harbor seal rRNA was removed using the Ribo-Zero Kit Gold (Human-Mouse-Rat) from Epicentre (WI, USA) following the manufacturer’s directions. High quality RNA was converted to cDNA using superscript II Reverse Transcriptase (Life Technologies, CA). Libraries were prepared for each of the 11 samples using the TruSeq paired end cluster kit v.3 from Illumina (San Diego, CA). Libraries were sequenced on two lanes of the Illumina Hi-Seq 2000 platform.Harbor seal brain transcriptome assembly was conducted using a combination of transcriptome guided and de novo methods. Eleven quality assured sequence libraries were combined and aligned to the hypothetical Weddell seal, Leptonychotes weddellii (NCBI accession: PRJNA232772) transcriptome, using the program Bowtie2-2.2.3 (21). Aligned sequences were used to build a de novo harbor seal transcriptome using Trinity 2.0.6 with parameters --single, and –full-cleanup. The longest representative transcript for each component or subcomponent in the transcriptome assembly was selected using trinity_reps.pl (https://goo.gl/EGq7I6 ). The National Marine Fisheries (NMFS) authorized the collection of tissue samples from stranded marine mammals and satisfies The Marine Mammal Protection Act (MMPA) regulation 50 CR 216.22 and 216.37. |
format |
Article in Journal/Newspaper |
author |
Rosales, Stephanie |
author_facet |
Rosales, Stephanie |
author_sort |
Rosales, Stephanie |
title |
Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
title_short |
Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
title_full |
Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
title_fullStr |
Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
title_full_unstemmed |
Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
title_sort |
brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection |
publisher |
figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.3581712.v1 https://figshare.com/articles/software/Brain_transcriptomes_of_harbor_seals_demonstrate_gene_expression_patterns_of_animals_undergoing_a_metabolic_disease_and_a_viral_infection/3581712/1 |
long_lat |
ENVELOPE(18.933,18.933,69.617,69.617) |
geographic |
Lanes Weddell |
geographic_facet |
Lanes Weddell |
genre |
harbor seal Phoca vitulina Weddell Seal |
genre_facet |
harbor seal Phoca vitulina Weddell Seal |
op_relation |
https://dx.doi.org/10.6084/m9.figshare.3581712 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.3581712.v1 https://doi.org/10.6084/m9.figshare.3581712 |
_version_ |
1766022790149832704 |