Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt
Runoff of metals represents one of the major environmental challenges related to historic and ongoing mining activity. In this study, transcriptomics (direct RNA sequencing [RNA-seq] and reverse-transcription quantitative polymerase chain reaction [RT-qPCR]) was used to predict toxicity of metal-ric...
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Taylor & Francis
2016
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ftdatacite:10.6084/m9.figshare.3510017 2023-05-15T15:31:12+02:00 Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt Olsvik, Pål A. Ulvund, John B. Teien, Hans C. Urke, Henning A. Lie, Kai K. Kristensen, Torstein 2016 https://dx.doi.org/10.6084/m9.figshare.3510017 https://tandf.figshare.com/articles/dataset/Transcriptional_effects_of_metal-rich_acid_drainage_water_from_the_abandoned_L_kken_Mine_on_Atlantic_salmon_i_Salmo_salar_i_smolt/3510017 unknown Taylor & Francis https://dx.doi.org/10.1080/15287394.2016.1171992 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biophysics Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry dataset Dataset 2016 ftdatacite https://doi.org/10.6084/m9.figshare.3510017 https://doi.org/10.1080/15287394.2016.1171992 2021-11-05T12:55:41Z Runoff of metals represents one of the major environmental challenges related to historic and ongoing mining activity. In this study, transcriptomics (direct RNA sequencing [RNA-seq] and reverse-transcription quantitative polymerase chain reaction [RT-qPCR]) was used to predict toxicity of metal-rich acid mine drainage (AMD) water collected in the abandoned copper (Cu) mine called Løkken Mine on Atlantic salmon liver and kidney, the main target organs of Cu-induced toxicity in fish. Smolts were exposed to control and diluted AMD water, which contains a mixture of metals but is especially enriched with Cu, at 4 concentrations in freshwater (FW) for 96 h, and then were transferred to and kept in seawater (SW) for another 24 h. Significant accumulation of Cu was observed in the gills, but not liver and kidney tissues, after 96 h of exposure. Short-term exposure to metal-rich ADM (high exposure group) significantly upregulated 3201 transcripts and downregulated 3782 transcripts in liver. The strongest effect attributed to exposure was observed on the KEGG pathway “protein processing in endoplasmic reticulum,” followed by “steroid biosynthesis.” Gene ontology (GO) analysis suggested that exposure predominantly affected “protein folding,” possibly by disrupting disulfide bonds as a result of endoplasmic-reticulum-generated stress, and “sterol biosynthetic processes.” Transfer to uncontaminated SW for 24 h amended the transcription of several genes, suggesting a transient effect of treatment on some mechanisms. In conclusion, the data show that trace metals in AMD from abandoned pyrite mines might disturb molecular mechanisms linked to protein folding in Atlantic salmon smolt endoplasmic reticulum. Dataset Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biophysics Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
spellingShingle |
Biophysics Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Olsvik, Pål A. Ulvund, John B. Teien, Hans C. Urke, Henning A. Lie, Kai K. Kristensen, Torstein Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt |
topic_facet |
Biophysics Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
description |
Runoff of metals represents one of the major environmental challenges related to historic and ongoing mining activity. In this study, transcriptomics (direct RNA sequencing [RNA-seq] and reverse-transcription quantitative polymerase chain reaction [RT-qPCR]) was used to predict toxicity of metal-rich acid mine drainage (AMD) water collected in the abandoned copper (Cu) mine called Løkken Mine on Atlantic salmon liver and kidney, the main target organs of Cu-induced toxicity in fish. Smolts were exposed to control and diluted AMD water, which contains a mixture of metals but is especially enriched with Cu, at 4 concentrations in freshwater (FW) for 96 h, and then were transferred to and kept in seawater (SW) for another 24 h. Significant accumulation of Cu was observed in the gills, but not liver and kidney tissues, after 96 h of exposure. Short-term exposure to metal-rich ADM (high exposure group) significantly upregulated 3201 transcripts and downregulated 3782 transcripts in liver. The strongest effect attributed to exposure was observed on the KEGG pathway “protein processing in endoplasmic reticulum,” followed by “steroid biosynthesis.” Gene ontology (GO) analysis suggested that exposure predominantly affected “protein folding,” possibly by disrupting disulfide bonds as a result of endoplasmic-reticulum-generated stress, and “sterol biosynthetic processes.” Transfer to uncontaminated SW for 24 h amended the transcription of several genes, suggesting a transient effect of treatment on some mechanisms. In conclusion, the data show that trace metals in AMD from abandoned pyrite mines might disturb molecular mechanisms linked to protein folding in Atlantic salmon smolt endoplasmic reticulum. |
format |
Dataset |
author |
Olsvik, Pål A. Ulvund, John B. Teien, Hans C. Urke, Henning A. Lie, Kai K. Kristensen, Torstein |
author_facet |
Olsvik, Pål A. Ulvund, John B. Teien, Hans C. Urke, Henning A. Lie, Kai K. Kristensen, Torstein |
author_sort |
Olsvik, Pål A. |
title |
Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt |
title_short |
Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt |
title_full |
Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt |
title_fullStr |
Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt |
title_full_unstemmed |
Transcriptional effects of metal-rich acid drainage water from the abandoned Løkken Mine on Atlantic salmon ( Salmo salar ) smolt |
title_sort |
transcriptional effects of metal-rich acid drainage water from the abandoned løkken mine on atlantic salmon ( salmo salar ) smolt |
publisher |
Taylor & Francis |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.3510017 https://tandf.figshare.com/articles/dataset/Transcriptional_effects_of_metal-rich_acid_drainage_water_from_the_abandoned_L_kken_Mine_on_Atlantic_salmon_i_Salmo_salar_i_smolt/3510017 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1080/15287394.2016.1171992 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.3510017 https://doi.org/10.1080/15287394.2016.1171992 |
_version_ |
1766361694570807296 |