Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves
Distinguishing morphologically cryptic taxa, by definition, requires genetic data such as DNA sequences. However, DNA sequences may not be obtained easily for taxa from remote sites. Here we provide the details of a high-resolution melt-curve-based method using taxon-specific primers that can distin...
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2016
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ftdatacite:10.6084/m9.figshare.3426569 2023-05-15T14:05:27+02:00 Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves JC Banks JA Clark P Nield J-AL Stanton E Wagner 2016 https://dx.doi.org/10.6084/m9.figshare.3426569 https://tandf.figshare.com/articles/dataset/Haplotyping_cryptic_Ad_lie_penguin_taxa_using_low-cost_high-resolution_melt_curves/3426569/1 unknown Taylor & Francis https://dx.doi.org/10.1080/03014223.2015.1129971 Creative Commons Attribution Non Commercial No Derivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode cc-by-nc-nd-4.0 CC-BY-NC-ND Biophysics Medicine Evolutionary Biology FOS Biological sciences Ecology Inorganic Chemistry FOS Chemical sciences Hematology 110309 Infectious Diseases FOS Health sciences 60506 Virology dataset Dataset 2016 ftdatacite https://doi.org/10.6084/m9.figshare.3426569 https://doi.org/10.1080/03014223.2015.1129971 2021-11-05T12:55:41Z Distinguishing morphologically cryptic taxa, by definition, requires genetic data such as DNA sequences. However, DNA sequences may not be obtained easily for taxa from remote sites. Here we provide the details of a high-resolution melt-curve-based method using taxon-specific primers that can distinguish two taxa of Adélie penguins, and that will be usable in Antarctica when combined with some of the newly developed field-deployable thermal cyclers. We suggest that the wider adoption of field-deployable polymerase-chain-reaction-based techniques will enable faster assignation of haplotype to individuals in situ, and so allow the targeting of observations and sample collection to specimens relevant to the research question. Targeting individuals will also reduce the need to repeatedly handle animals and reduce the time and travel required to complete field work. Dataset Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biophysics Medicine Evolutionary Biology FOS Biological sciences Ecology Inorganic Chemistry FOS Chemical sciences Hematology 110309 Infectious Diseases FOS Health sciences 60506 Virology |
spellingShingle |
Biophysics Medicine Evolutionary Biology FOS Biological sciences Ecology Inorganic Chemistry FOS Chemical sciences Hematology 110309 Infectious Diseases FOS Health sciences 60506 Virology JC Banks JA Clark P Nield J-AL Stanton E Wagner Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves |
topic_facet |
Biophysics Medicine Evolutionary Biology FOS Biological sciences Ecology Inorganic Chemistry FOS Chemical sciences Hematology 110309 Infectious Diseases FOS Health sciences 60506 Virology |
description |
Distinguishing morphologically cryptic taxa, by definition, requires genetic data such as DNA sequences. However, DNA sequences may not be obtained easily for taxa from remote sites. Here we provide the details of a high-resolution melt-curve-based method using taxon-specific primers that can distinguish two taxa of Adélie penguins, and that will be usable in Antarctica when combined with some of the newly developed field-deployable thermal cyclers. We suggest that the wider adoption of field-deployable polymerase-chain-reaction-based techniques will enable faster assignation of haplotype to individuals in situ, and so allow the targeting of observations and sample collection to specimens relevant to the research question. Targeting individuals will also reduce the need to repeatedly handle animals and reduce the time and travel required to complete field work. |
format |
Dataset |
author |
JC Banks JA Clark P Nield J-AL Stanton E Wagner |
author_facet |
JC Banks JA Clark P Nield J-AL Stanton E Wagner |
author_sort |
JC Banks |
title |
Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves |
title_short |
Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves |
title_full |
Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves |
title_fullStr |
Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves |
title_full_unstemmed |
Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves |
title_sort |
haplotyping cryptic adélie penguin taxa using low-cost, high-resolution melt curves |
publisher |
Taylor & Francis |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.3426569 https://tandf.figshare.com/articles/dataset/Haplotyping_cryptic_Ad_lie_penguin_taxa_using_low-cost_high-resolution_melt_curves/3426569/1 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.1080/03014223.2015.1129971 |
op_rights |
Creative Commons Attribution Non Commercial No Derivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode cc-by-nc-nd-4.0 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.6084/m9.figshare.3426569 https://doi.org/10.1080/03014223.2015.1129971 |
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1766277362465374208 |