Genomic context determines the effect of DNA methylation on gene expression in the gut epithelium of Atlantic salmon ( Salmo salar ) ...

• Main Authors: Katirtzoglou, Aikaterini, Hansen, Søren B., Sveier, Harald, Martin, Michael D., Brealey, Jaelle C., Limborg, Morten T.
• Format: Dataset
• Language: unknown
• Published: Taylor & Francis 2024
• Subjects: Cell Biology; Genetics; FOS: Biological sciences; Molecular Biology; Biological Sciences not elsewhere classified; Plant Biology; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.26767651; https://tandf.figshare.com/articles/dataset/Genomic_context_determines_the_effect_of_DNA_methylation_on_gene_expression_in_the_gut_epithelium_of_Atlantic_salmon_i_Salmo_salar_i_/26767651

The canonical view of DNA methylation, a pivotal epigenetic regulation mechanism in eukaryotes, dictates its role as a suppressor of gene activity, particularly within promoter regions. However, this view is being challenged as it is becoming increasingly evident that the connection between DNA methylation and gene expression varies depending on the genomic location and is therefore more complex than initially thought. We examined DNA methylation levels in the gut epithelium of Atlantic salmon ( Salmo salar ) using whole-genome bisulfite sequencing, which we correlated with gene expression data from RNA sequencing of the same gut tissue sample (RNA-seq). Assuming epigenetic signals might be pronounced between distinctive phenotypes, we compared large and small fish, finding 22 significant associations between 22 differentially methylated regions and 21 genes. We did not detect significant methylation differences between large and small fish. However, we observed a consistent signal of methylation levels ...