Atlantic Puffin Fecal DNA Metabarcoding: 2021-2022, Maine, USA ...

• Main Authors: Kennerley, William, Clucas, Gemma V., E. Lyons, Donald
• Format: Dataset
• Language: unknown
• Published: figshare 2024
• Subjects: Marine and estuarine ecology incl. marine ichthyology; Atlantic puffin; fratercula; Fratercula arctica
• Online Access: https://dx.doi.org/10.6084/m9.figshare.25365754; https://figshare.com/articles/dataset/Atlantic_Puffin_Fecal_DNA_Metabarcoding_2021-2022_Maine_USA/25365754

AbstractThese data include quantifications of prey types identified through metabarcoding using 12S and 18S genes and the DNA present in Atlantic puffin (Fratercula arctica) feces . Fecal samples were collected on Matinicus Rock, Maine during the incubation and chick-rearing periods of 2021 and 2022. Fecal samples were stored in DNA/RNA shield and were processed within 12 weeks of collection, with processing performed at the Cornell Lab of Ornithology and sequencing performed on an Illumina Hi-seq 2500 at the University of New Hampshire. Samples have been rarefied to an even read depth (600 reads for 12S, 2500 for 18S) and all bioinformatics work was performed by GVC in Qiime2. Data are presented here in separate csv files for each year and gene region examined, as well as corresponding metadata relating to sample collection (date and age of bird).Subject keywordsBiological sciences, DNA metabarcoding, Fratercula arctica, Gulf of Maine, seabird dietREADME: Atlantic Puffin Fecal DNA Metabarcoding: 2021-2022, ...