Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ...
Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential...
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ftdatacite:10.6084/m9.figshare.24220342 2023-12-03T10:13:26+01:00 Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... York, Julia M. 2023 https://dx.doi.org/10.6084/m9.figshare.24220342 https://rs.figshare.com/articles/journal_contribution/Supplementary_material_and_figures_from_Temperature_activated_transient_receptor_potential_ion_channels_from_Antarctic_fishes/24220342 unknown The Royal Society https://dx.doi.org/10.1098/rsob.20230215 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Plant Cell and Molecular Biology Animal Cell and Molecular Biology ScholarlyArticle Text article-journal Journal contribution 2023 ftdatacite https://doi.org/10.6084/m9.figshare.2422034210.1098/rsob.20230215 2023-11-03T10:38:11Z Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential (TRP) channels TRPA1b, TRPM4 and TRPV1a as the top candidates for temperature sensors. Here, cryonotothenioid TRPA1b and TRPV1a are characterized using Xenopus oocyte electrophysiology. TRPA1b and TRPV1a showed heat-evoked currents with Q10s of 11.1 ± 2.2 and 20.5 ± 2.4, respectively. Unexpectedly, heat activation occurred at a threshold of 22.9 ± 1.3°C for TRPA1b and 32.1 ± 0.6°C for TRPV1a. These fish have not experienced such temperatures for at least 15 Myr. Either (1) another molecular mechanism underlies temperature sensation, (2) these fishes do not sense temperatures below these thresholds despite having lethal limits as low as 5°C, or (3) native cellular conditions modify the TRP channels to function at ... Other Non-Article Part of Journal/Newspaper Antarc* Antarctic DataCite Metadata Store (German National Library of Science and Technology) Antarctic |
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Plant Cell and Molecular Biology Animal Cell and Molecular Biology |
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Plant Cell and Molecular Biology Animal Cell and Molecular Biology York, Julia M. Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... |
topic_facet |
Plant Cell and Molecular Biology Animal Cell and Molecular Biology |
description |
Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential (TRP) channels TRPA1b, TRPM4 and TRPV1a as the top candidates for temperature sensors. Here, cryonotothenioid TRPA1b and TRPV1a are characterized using Xenopus oocyte electrophysiology. TRPA1b and TRPV1a showed heat-evoked currents with Q10s of 11.1 ± 2.2 and 20.5 ± 2.4, respectively. Unexpectedly, heat activation occurred at a threshold of 22.9 ± 1.3°C for TRPA1b and 32.1 ± 0.6°C for TRPV1a. These fish have not experienced such temperatures for at least 15 Myr. Either (1) another molecular mechanism underlies temperature sensation, (2) these fishes do not sense temperatures below these thresholds despite having lethal limits as low as 5°C, or (3) native cellular conditions modify the TRP channels to function at ... |
format |
Other Non-Article Part of Journal/Newspaper |
author |
York, Julia M. |
author_facet |
York, Julia M. |
author_sort |
York, Julia M. |
title |
Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... |
title_short |
Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... |
title_full |
Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... |
title_fullStr |
Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... |
title_full_unstemmed |
Supplementary material and figures from Temperature activated transient receptor potential ion channels from Antarctic fishes ... |
title_sort |
supplementary material and figures from temperature activated transient receptor potential ion channels from antarctic fishes ... |
publisher |
The Royal Society |
publishDate |
2023 |
url |
https://dx.doi.org/10.6084/m9.figshare.24220342 https://rs.figshare.com/articles/journal_contribution/Supplementary_material_and_figures_from_Temperature_activated_transient_receptor_potential_ion_channels_from_Antarctic_fishes/24220342 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://dx.doi.org/10.1098/rsob.20230215 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.2422034210.1098/rsob.20230215 |
_version_ |
1784260193042825216 |