The dark art of cultivating glacier ice algae ...

• Main Authors: Jensen, Marie Bolander, Perini, Laura, Halbach, Laura, Jakobsen, Hans, Haraguchi, Lumi, Ribeiro, Sofia, Tranter, Martyn, Benning, Liane G., Anesio, Alexandre M.
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: Taylor & Francis 2023
• Subjects: Medicine; Microbiology; FOS Biological sciences; Environmental Sciences not elsewhere classified; Ecology; Biological Sciences not elsewhere classified; Plant Biology; ice algae
• Online Access: https://dx.doi.org/10.6084/m9.figshare.24091430.v1; https://tandf.figshare.com/articles/journal_contribution/The_dark_art_of_cultivating_glacier_ice_algae/24091430/1

The Ancylonema genus includes the most-documented microalgae on glaciers and ice sheets worldwide. There is significant interest in these microalgae in the context of climate change, considering their role in lowering surface ice albedo and acceleration of ice melt. However, currently, no cultures of the two closely related species A. nordenskiöldii or A. alaskanum have been established, restricting our ability to study these globally important species under laboratory conditions. We established and kept cultures of Ancylonema sp. alive for up to 2 years, by testing and optimizing different growth media and parameters. Maximum growth was achieved when using 1:100 diluted media with soil extract and low light intensity (300 µmol m −2 s −1 ). However, as a consequence of incubation in lab conditions, some of the cultures lost their purpurogallin pigmentation and appeared green. Sanger sequencing of the ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit ( rbc L) marker gene revealed a large genetic ...