Additional file 3 of Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights ...

• Main Authors: Poisson, William, Prunier, Julien, Carrier, Alexandra, Gilbert, Isabelle, Mastromonaco, Gabriela, Albert, Vicky, Taillon, Joëlle, Bourret, Vincent, Droit, Arnaud, Côté, Steeve D., Robert, Claude
• Format: Dataset
• Language: unknown
• Published: figshare 2023
• Subjects: Genetics; FOS Biological sciences; Rangifer tarandus
• Online Access: https://dx.doi.org/10.6084/m9.figshare.22621945; https://springernature.figshare.com/articles/dataset/Additional_file_3_of_Chromosome-level_assembly_of_the_Rangifer_tarandus_genome_and_validation_of_cervid_and_bovid_evolution_insights/22621945

Additional file 3. Primers and detection oligo. Excel file listing the primers used for amplification and transcription reactions and as detection oligo. Reverse primers consist of the 20-mers complementary sequence linked to the 3’ end of the T7 sequence 5’-CGATTGAGGCCGGTAATACGACTCACTATAGGG-3’ [45]. Forward primer 20-mers are linked to the 3’ end of the adapter sequence. The resulting 53-mers reverse primer and 40-mers forward primer oligo were used for PCR. Forward primers were used also for RT-PCR. The fluorophore-linked homolog of the specific 20-mers adapter was the detector of probe binding. These 30 oligo were purchased from IDT as custom standard or HPLC-purified DNA. ...