Cross-species infection potential of avian influenza H13 viruses isolated from wild aquatic birds to poultry and mammals ...

• Main Authors: Sun, Weiyang, Zhao, Menglin, Yu, Zhijun, Li, Yuanguo, Zhang, Xinghai, Feng, Na, Wang, Tiecheng, Wang, Hongmei, He, Hongbin, Zhao, Yongkun, Yang, Songtao, Xia, Xianzhu, Gao, Yuwei
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Taylor & Francis 2023
• Subjects: Genetics; FOS Biological sciences; Neuroscience; Biotechnology; Evolutionary Biology; Environmental Sciences not elsewhere classified; Ecology; Immunology; FOS Clinical medicine; Biological Sciences not elsewhere classified; Cancer; Darby; Eurasian Curlew
• Online Access: https://dx.doi.org/10.6084/m9.figshare.22220643.v1; https://tandf.figshare.com/articles/online_resource/Cross-species_infection_potential_of_avian_influenza_H13_viruses_isolated_from_wild_aquatic_birds_to_poultry_and_mammals/22220643/1

Wild aquatic birds are the primary hosts of H13 avian influenza viruses (AIVs). Herein, we performed a genetic analysis of two H13 AIVs isolated from wild birds in China and evaluated their infection potential in poultry to further explore the potential for transmission from wild aquatic birds to poultry. Our results showed that the two strains belong to different groups, one strain (A/mallard/Dalian/DZ-137/2013; abbreviated as DZ137) belongs to Group I, whereas the other strain (A/Eurasian Curlew/Liaoning/ZH-385/2014; abbreviated as ZH385) belongs to Group III. In vitro experiments showed that both DZ137 and ZH385 can replicate efficiently in chicken embryo fibroblast cells. We found that these H13 AIVs can also efficiently replicate in mammalian cell lines, including human embryonic kidney cells and Madin-Darby canine kidney cells. In vivo experiments showed that DZ137 and ZH385 can infect 1-day-old specific pathogen-free (SPF) chickens, and that ZH385 has a higher replication ability in chickens than ...