BLASTN Analysis Results Files

• Main Authors: Lofts, Brandon, Ramirez, Elizabeth, vasquez, delbert, Tang, Xiaolu
• Format: Dataset
• Language: unknown
• Published: figshare 2022
• Subjects: 60102 Bioinformatics; FOS Computer and information sciences; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.19333238.v1; https://figshare.com/articles/dataset/BLASTN_Analysis_Results_Files/19333238/1

BLASTN Analysis Results Files The Salmo salar soluble Toll-like receptor 5 (sTLR5) sequence was found in the Stephen et al. paper with a linked accession number. This accession number took us to the NIH website where we were able to download the CDS sequence of the sTLR5 as a FASTA file. The Salmo salar sTLR5 was then used to run a BLASTN analysis against each species cDNA on Ensembl Genome Browser 105. The search sensitivity was set to "Normal." We then identified hits with a high percent Identity and an e-value of 0.0, making sure that the hits were in fact different genes (different subject name and location in genome). These hits were then saved as a FASTA using their CDS. They were then uploaded into AliView and aligned using MUSCLE.