rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean
Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions...
Main Authors: | , , , , , , |
---|---|
Format: | Text |
Language: | unknown |
Published: |
Taylor & Francis
2015
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.1582663.v1 https://tandf.figshare.com/articles/rRNA_and_rDNA_based_assessment_of_sea_ice_protist_biodiversity_from_the_central_Arctic_Ocean/1582663/1 |
id |
ftdatacite:10.6084/m9.figshare.1582663.v1 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.1582663.v1 2023-05-15T14:50:21+02:00 rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Bánk Beszteri Kroth, Peter G. Valentin, Klaus 2015 https://dx.doi.org/10.6084/m9.figshare.1582663.v1 https://tandf.figshare.com/articles/rRNA_and_rDNA_based_assessment_of_sea_ice_protist_biodiversity_from_the_central_Arctic_Ocean/1582663/1 unknown Taylor & Francis https://dx.doi.org/10.1080/09670262.2015.1077395 https://dx.doi.org/10.6084/m9.figshare.1582663 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Microbiology FOS Biological sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Marine Biology Inorganic Chemistry Text article-journal Journal contribution ScholarlyArticle 2015 ftdatacite https://doi.org/10.6084/m9.figshare.1582663.v1 https://doi.org/10.1080/09670262.2015.1077395 https://doi.org/10.6084/m9.figshare.1582663 2021-11-05T12:55:41Z Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions; as a consequence, summer sea ice cover is decreasing and MYI is disappearing at an alarming rate. Despite its apparent hostility, sea ice is inhabited by a diverse microbial community of bacteria and protists, many of which are photosynthetic. Here we present an assessment of eukaryotic biodiversity in MYI and FYI from the central Arctic Ocean using high-throughput 454 sequencing of 18S rRNA and rDNA amplicons. We compared the rDNA-based ‘total’ biodiversity with the ‘active’ biodiversity from rRNA amplicons and found differences between them including an over-representation of Ciliophora, Bicosoecida and Bacillariophyceae operational taxonomic units (OTUs) in the active part of the community. Differences between the two libraries are more pronounced at the lower taxonomic level: certain genera, such as Melosira , are more abundant in the rRNA library, indicating activity of these genera. Furthermore, we found that one FYI station showed a higher activity of potential grazers which was probably due to the advanced stage of melt evident by higher ice temperatures and highly porous ice compared with the other stations. Text Arctic Arctic Ocean Global warming Sea ice DataCite Metadata Store (German National Library of Science and Technology) Arctic Arctic Ocean |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Microbiology FOS Biological sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Marine Biology Inorganic Chemistry |
spellingShingle |
Microbiology FOS Biological sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Marine Biology Inorganic Chemistry Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Bánk Beszteri Kroth, Peter G. Valentin, Klaus rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
topic_facet |
Microbiology FOS Biological sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Marine Biology Inorganic Chemistry |
description |
Sea ice is a large and diverse ecosystem contributing significantly to primary production in ice-covered regions. In the Arctic Ocean, sea ice consists of mixed multi-year ice (MYI), often several metres thick, and thinner first-year ice (FYI). Current global warming is most severe in Arctic regions; as a consequence, summer sea ice cover is decreasing and MYI is disappearing at an alarming rate. Despite its apparent hostility, sea ice is inhabited by a diverse microbial community of bacteria and protists, many of which are photosynthetic. Here we present an assessment of eukaryotic biodiversity in MYI and FYI from the central Arctic Ocean using high-throughput 454 sequencing of 18S rRNA and rDNA amplicons. We compared the rDNA-based ‘total’ biodiversity with the ‘active’ biodiversity from rRNA amplicons and found differences between them including an over-representation of Ciliophora, Bicosoecida and Bacillariophyceae operational taxonomic units (OTUs) in the active part of the community. Differences between the two libraries are more pronounced at the lower taxonomic level: certain genera, such as Melosira , are more abundant in the rRNA library, indicating activity of these genera. Furthermore, we found that one FYI station showed a higher activity of potential grazers which was probably due to the advanced stage of melt evident by higher ice temperatures and highly porous ice compared with the other stations. |
format |
Text |
author |
Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Bánk Beszteri Kroth, Peter G. Valentin, Klaus |
author_facet |
Stecher, Anique Neuhaus, Stefan Lange, Benjamin Frickenhaus, Stephan Bánk Beszteri Kroth, Peter G. Valentin, Klaus |
author_sort |
Stecher, Anique |
title |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_short |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_full |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_fullStr |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_full_unstemmed |
rRNA and rDNA based assessment of sea ice protist biodiversity from the central Arctic Ocean |
title_sort |
rrna and rdna based assessment of sea ice protist biodiversity from the central arctic ocean |
publisher |
Taylor & Francis |
publishDate |
2015 |
url |
https://dx.doi.org/10.6084/m9.figshare.1582663.v1 https://tandf.figshare.com/articles/rRNA_and_rDNA_based_assessment_of_sea_ice_protist_biodiversity_from_the_central_Arctic_Ocean/1582663/1 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Global warming Sea ice |
genre_facet |
Arctic Arctic Ocean Global warming Sea ice |
op_relation |
https://dx.doi.org/10.1080/09670262.2015.1077395 https://dx.doi.org/10.6084/m9.figshare.1582663 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.1582663.v1 https://doi.org/10.1080/09670262.2015.1077395 https://doi.org/10.6084/m9.figshare.1582663 |
_version_ |
1766321383621525504 |