Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period
Deep polar ice cores provide atmospheric records of nitrous oxide (N 2 O) and other trace gases reflecting climate history along with a parallel archive of microbial cells transported with mineral dust, marine and volcanic aerosols from around the globe. Our interdisciplinary study of 32 samples fro...
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Taylor & Francis
2016
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ftdatacite:10.6084/m9.figshare.1568465.v3 2023-05-15T16:28:38+02:00 Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period Miteva, Vanya Sowers, Todd Schüpbach, Simon Fischer, Hubertus Brenchley, Jean 2016 https://dx.doi.org/10.6084/m9.figshare.1568465.v3 https://tandf.figshare.com/articles/dataset/Geochemical_and_Microbiological_Studies_of_Nitrous_Oxide_Variations_within_the_New_NEEM_Greenland_Ice_Core_during_the_Last_Glacial_Period/1568465/3 unknown Taylor & Francis https://dx.doi.org/10.1080/01490451.2015.1074321 https://dx.doi.org/10.6084/m9.figshare.1568465 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Microbiology FOS Biological sciences Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry dataset Dataset 2016 ftdatacite https://doi.org/10.6084/m9.figshare.1568465.v3 https://doi.org/10.1080/01490451.2015.1074321 https://doi.org/10.6084/m9.figshare.1568465 2021-11-05T12:55:41Z Deep polar ice cores provide atmospheric records of nitrous oxide (N 2 O) and other trace gases reflecting climate history along with a parallel archive of microbial cells transported with mineral dust, marine and volcanic aerosols from around the globe. Our interdisciplinary study of 32 samples from different depths of the recently drilled NEEM Greenland ice core addressed the question whether the identified microorganisms were capable of post-depositional biological production of N 2 O in situ. We used high-resolution geochemical and microbiological approaches to examine the N 2 O concentrations, the quantitative distributions of dust, Ca +2 , NH 4 + and NO 3 − ions related to N cycle pathways, the microbial abundance and diversity at specific NEEM core depths from 1758 m to 1867.8 m. Results showed varying concentrations of N 2 O (220–271.5 ppb). Microbial abundance fluctuated between 3.3 × 10 4 and 3.3 × 10 6 cells mL −1 in direct correlation with dust and Ca 2+ concentrations with higher cell numbers deposited during colder periods. The average values of NH 4 + and NO 3 − indicated that substrates were available for the microorganisms capable of utilizing them. PCR amplification of selected functional genes involved in bacterial and archaeal nitrification and denitrification was not successful. Sanger and Illumina MiSeq sequence analyses of SSU rRNA genes showed variable representation of A lpha-, Beta- and Gammaproteobacteria, Firmicutes, Actinobacteria , chloroplasts and fungi. The metabolic potential of the dominant genera of Proteobacteria and Firmicutes as possible N 2 O producers suggested that denitrification activity may have led to in-situ production and accumulation of N 2 O. Dataset Greenland Greenland ice core ice core DataCite Metadata Store (German National Library of Science and Technology) Greenland |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Microbiology FOS Biological sciences Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry |
spellingShingle |
Microbiology FOS Biological sciences Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry Miteva, Vanya Sowers, Todd Schüpbach, Simon Fischer, Hubertus Brenchley, Jean Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period |
topic_facet |
Microbiology FOS Biological sciences Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry |
description |
Deep polar ice cores provide atmospheric records of nitrous oxide (N 2 O) and other trace gases reflecting climate history along with a parallel archive of microbial cells transported with mineral dust, marine and volcanic aerosols from around the globe. Our interdisciplinary study of 32 samples from different depths of the recently drilled NEEM Greenland ice core addressed the question whether the identified microorganisms were capable of post-depositional biological production of N 2 O in situ. We used high-resolution geochemical and microbiological approaches to examine the N 2 O concentrations, the quantitative distributions of dust, Ca +2 , NH 4 + and NO 3 − ions related to N cycle pathways, the microbial abundance and diversity at specific NEEM core depths from 1758 m to 1867.8 m. Results showed varying concentrations of N 2 O (220–271.5 ppb). Microbial abundance fluctuated between 3.3 × 10 4 and 3.3 × 10 6 cells mL −1 in direct correlation with dust and Ca 2+ concentrations with higher cell numbers deposited during colder periods. The average values of NH 4 + and NO 3 − indicated that substrates were available for the microorganisms capable of utilizing them. PCR amplification of selected functional genes involved in bacterial and archaeal nitrification and denitrification was not successful. Sanger and Illumina MiSeq sequence analyses of SSU rRNA genes showed variable representation of A lpha-, Beta- and Gammaproteobacteria, Firmicutes, Actinobacteria , chloroplasts and fungi. The metabolic potential of the dominant genera of Proteobacteria and Firmicutes as possible N 2 O producers suggested that denitrification activity may have led to in-situ production and accumulation of N 2 O. |
format |
Dataset |
author |
Miteva, Vanya Sowers, Todd Schüpbach, Simon Fischer, Hubertus Brenchley, Jean |
author_facet |
Miteva, Vanya Sowers, Todd Schüpbach, Simon Fischer, Hubertus Brenchley, Jean |
author_sort |
Miteva, Vanya |
title |
Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period |
title_short |
Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period |
title_full |
Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period |
title_fullStr |
Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period |
title_full_unstemmed |
Geochemical and Microbiological Studies of Nitrous Oxide Variations within the New NEEM Greenland Ice Core during the Last Glacial Period |
title_sort |
geochemical and microbiological studies of nitrous oxide variations within the new neem greenland ice core during the last glacial period |
publisher |
Taylor & Francis |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.1568465.v3 https://tandf.figshare.com/articles/dataset/Geochemical_and_Microbiological_Studies_of_Nitrous_Oxide_Variations_within_the_New_NEEM_Greenland_Ice_Core_during_the_Last_Glacial_Period/1568465/3 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Greenland ice core ice core |
genre_facet |
Greenland Greenland ice core ice core |
op_relation |
https://dx.doi.org/10.1080/01490451.2015.1074321 https://dx.doi.org/10.6084/m9.figshare.1568465 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.1568465.v3 https://doi.org/10.1080/01490451.2015.1074321 https://doi.org/10.6084/m9.figshare.1568465 |
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1766018315463950336 |