Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors
Additional file 5: Figure S4. Illustration of the settings applied for filtering, trimming and variant calling of the MiSeq reads. Fastq files were filtered and trimmed with the following specifications; primer sequences were used to demultiplex reads from different amplicons on the same sequencing...
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ftdatacite:10.6084/m9.figshare.15041181 2023-05-15T15:32:08+02:00 Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik 2021 https://dx.doi.org/10.6084/m9.figshare.15041181 https://springernature.figshare.com/articles/presentation/Additional_file_5_of_Single_nucleotide_replacement_in_the_Atlantic_salmon_genome_using_CRISPR_Cas9_and_asymmetrical_oligonucleotide_donors/15041181 unknown figshare https://dx.doi.org/10.1186/s12864-021-07823-8 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Presentation MediaObject article Audiovisual 2021 ftdatacite https://doi.org/10.6084/m9.figshare.15041181 https://doi.org/10.1186/s12864-021-07823-8 2021-11-05T12:55:41Z Additional file 5: Figure S4. Illustration of the settings applied for filtering, trimming and variant calling of the MiSeq reads. Fastq files were filtered and trimmed with the following specifications; primer sequences were used to demultiplex reads from different amplicons on the same sequencing run, minimum read length was set to 100 bp, and forward and reverse reads were assembled to correct sequencing errors (minimum overlap between forward and reverse reads was set to 150 bp for slc45a2 and 200 bp for dnd and allowing at most 20% mismatches between forward and reverse reads in the overlap region). Assembled reads were combined with forward reads that did not pass the assembly thresholds. Variants were then called using positions 20–200 for slc45a2 and positions 60–230 for dnd. All bases with base quality Conference Object Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences |
spellingShingle |
Genetics FOS Biological sciences Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
topic_facet |
Genetics FOS Biological sciences |
description |
Additional file 5: Figure S4. Illustration of the settings applied for filtering, trimming and variant calling of the MiSeq reads. Fastq files were filtered and trimmed with the following specifications; primer sequences were used to demultiplex reads from different amplicons on the same sequencing run, minimum read length was set to 100 bp, and forward and reverse reads were assembled to correct sequencing errors (minimum overlap between forward and reverse reads was set to 150 bp for slc45a2 and 200 bp for dnd and allowing at most 20% mismatches between forward and reverse reads in the overlap region). Assembled reads were combined with forward reads that did not pass the assembly thresholds. Variants were then called using positions 20–200 for slc45a2 and positions 60–230 for dnd. All bases with base quality |
format |
Conference Object |
author |
Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik |
author_facet |
Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik |
author_sort |
Straume, Anne Hege |
title |
Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_short |
Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_full |
Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_fullStr |
Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_full_unstemmed |
Additional file 5 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_sort |
additional file 5 of single nucleotide replacement in the atlantic salmon genome using crispr/cas9 and asymmetrical oligonucleotide donors |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.15041181 https://springernature.figshare.com/articles/presentation/Additional_file_5_of_Single_nucleotide_replacement_in_the_Atlantic_salmon_genome_using_CRISPR_Cas9_and_asymmetrical_oligonucleotide_donors/15041181 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1186/s12864-021-07823-8 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.15041181 https://doi.org/10.1186/s12864-021-07823-8 |
_version_ |
1766362645656502272 |