Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors
Additional file 3: Figure S2. Comparison of symmetrical and asymmetrical ODNs for slc45a2 FLAG knock-in. All the ODNs compared here were designed for slc45a2 to KI a FLAG element. The ODN concentration was 1.5 μM. The symmetrical ODNs are a pool of S 24, AS 24, ds 24, S 48 and S 84 (described in Str...
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Online Access: | https://dx.doi.org/10.6084/m9.figshare.15041175 https://springernature.figshare.com/articles/presentation/Additional_file_3_of_Single_nucleotide_replacement_in_the_Atlantic_salmon_genome_using_CRISPR_Cas9_and_asymmetrical_oligonucleotide_donors/15041175 |
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ftdatacite:10.6084/m9.figshare.15041175 2023-05-15T15:32:15+02:00 Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik 2021 https://dx.doi.org/10.6084/m9.figshare.15041175 https://springernature.figshare.com/articles/presentation/Additional_file_3_of_Single_nucleotide_replacement_in_the_Atlantic_salmon_genome_using_CRISPR_Cas9_and_asymmetrical_oligonucleotide_donors/15041175 unknown figshare https://dx.doi.org/10.1186/s12864-021-07823-8 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Presentation MediaObject article Audiovisual 2021 ftdatacite https://doi.org/10.6084/m9.figshare.15041175 https://doi.org/10.1186/s12864-021-07823-8 2021-11-05T12:55:41Z Additional file 3: Figure S2. Comparison of symmetrical and asymmetrical ODNs for slc45a2 FLAG knock-in. All the ODNs compared here were designed for slc45a2 to KI a FLAG element. The ODN concentration was 1.5 μM. The symmetrical ODNs are a pool of S 24, AS 24, ds 24, S 48 and S 84 (described in Straume et.al 2020). The asymmetrical ODN design is illustrated in Suplemmentary Fig. 1 A. Green dots represent perfect HDR, black squares represent erroneous HDR. Mutant fish were analysed using Illumina MiSeq. Read counts for each sample are given in % of the total number of reads with at least 100 identical reads. The error bars indicate the SEM of the mean for each group. A Mann-Whitney test was used to compare the mean rank of symmetrical vs. asymmetrical ODNs, analyzing the groups perfect and erroneous HDR separately. Different lower-case letters indicate significant differences (P Conference Object Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) Straume ENVELOPE(14.472,14.472,68.689,68.689) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Genetics FOS Biological sciences |
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Genetics FOS Biological sciences Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
topic_facet |
Genetics FOS Biological sciences |
description |
Additional file 3: Figure S2. Comparison of symmetrical and asymmetrical ODNs for slc45a2 FLAG knock-in. All the ODNs compared here were designed for slc45a2 to KI a FLAG element. The ODN concentration was 1.5 μM. The symmetrical ODNs are a pool of S 24, AS 24, ds 24, S 48 and S 84 (described in Straume et.al 2020). The asymmetrical ODN design is illustrated in Suplemmentary Fig. 1 A. Green dots represent perfect HDR, black squares represent erroneous HDR. Mutant fish were analysed using Illumina MiSeq. Read counts for each sample are given in % of the total number of reads with at least 100 identical reads. The error bars indicate the SEM of the mean for each group. A Mann-Whitney test was used to compare the mean rank of symmetrical vs. asymmetrical ODNs, analyzing the groups perfect and erroneous HDR separately. Different lower-case letters indicate significant differences (P |
format |
Conference Object |
author |
Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik |
author_facet |
Straume, Anne Hege Kjærner-Semb, Erik Skaftnesmo, Kai Ove Güralp, Hilal Lillico, Simon Wargelius, Anna Edvardsen, Rolf Brudvik |
author_sort |
Straume, Anne Hege |
title |
Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_short |
Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_full |
Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_fullStr |
Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_full_unstemmed |
Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors |
title_sort |
additional file 3 of single nucleotide replacement in the atlantic salmon genome using crispr/cas9 and asymmetrical oligonucleotide donors |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.15041175 https://springernature.figshare.com/articles/presentation/Additional_file_3_of_Single_nucleotide_replacement_in_the_Atlantic_salmon_genome_using_CRISPR_Cas9_and_asymmetrical_oligonucleotide_donors/15041175 |
long_lat |
ENVELOPE(14.472,14.472,68.689,68.689) |
geographic |
Straume |
geographic_facet |
Straume |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1186/s12864-021-07823-8 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.15041175 https://doi.org/10.1186/s12864-021-07823-8 |
_version_ |
1766362761401466880 |