High-throughput sequencing of the kelp Alaria (Phaeophyceae) reveals epi-endobiotic associations, including a likely phaeophycean parasite ...

• Main Authors: Bringloe, Trevor T., Sauermann, Ryan, Krause-Jensen, Dorte, Olesen, Birgit, Klimova, Anna, Klochkova, Tatyana A., Verbruggen, Heroen
• Format: Text
• Language: unknown
• Published: Taylor & Francis 2021
• Subjects: Microbiology; FOS Biological sciences; Genetics; Evolutionary Biology; Ecology; Biological Sciences not elsewhere classified; Infectious Diseases; FOS Health sciences; Virology; Canada; Greenland; Nuuk; Kamchatka
• Online Access: https://dx.doi.org/10.6084/m9.figshare.14402189; https://tandf.figshare.com/articles/journal_contribution/High-throughput_sequencing_of_the_kelp_i_Alaria_i_Phaeophyceae_reveals_epi-endobiotic_associations_including_a_likely_phaeophycean_parasite/14402189

Whole genome sequencing datasets present the opportunity to not only study evolution in the target organism, but also the associated holobiont. The capacity to study epi-endobiotic kelp associations is improving substantially with the increased availability of high-throughput sequencing datasets. The goal of this study was to determine if shotgun sequencing libraries could be used to document epi- and endophyte/faunal species colonizing Alaria kelp sporophytes from Kamchatka (Russia), the Bay of Fundy (Atlantic Canada) and Nuuk (Greenland). Mitochondrial cox I and plastid rbc L reads were extracted and assembled from six Alaria whole genome sequencing datasets. In total, contigs representing 11 epi-endobiotic species were assembled, of which Chordariacean diversity dominated. Given the presence of a newly discovered phaeophycean cox I sequence lacking an rbc L counterpart, we secondarily tested our hypothesis that the cox I sequence belonged to a phaeophycean parasite. The entire read dataset was assembled ...