The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ...
The Pacific oyster Crassostrea gigas is a potential model organism of bivalve mollusks. Comprehensive studies on the proteome of its sperm are necessary to expand our understanding on its reproduction and development, which however are still poor currently. In this study, to improve the situation, w...
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ftdatacite:10.6084/m9.figshare.1419594 2024-04-28T08:16:26+00:00 The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... Huang, Xiaohong Liu, Baozhong Huan, Pin 2015 https://dx.doi.org/10.6084/m9.figshare.1419594 https://tandf.figshare.com/articles/journal_contribution/The_sperm_proteome_of_the_Pacific_oyster_i_Crassostrea_gigas_i_and_immunolocalization_of_heat_shock_proteins/1419594 unknown Taylor & Francis https://dx.doi.org/10.1080/07924259.2015.1041654 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Hematology Inorganic Chemistry FOS Chemical sciences Developmental Biology Biological Sciences Earth and Environmental Sciences Evolutionary Biology FOS Biological sciences Molecular Biology Cell Biology Biochemistry Biophysics Journal contribution article-journal Text ScholarlyArticle 2015 ftdatacite https://doi.org/10.6084/m9.figshare.141959410.1080/07924259.2015.1041654 2024-04-02T12:12:48Z The Pacific oyster Crassostrea gigas is a potential model organism of bivalve mollusks. Comprehensive studies on the proteome of its sperm are necessary to expand our understanding on its reproduction and development, which however are still poor currently. In this study, to improve the situation, we conducted a proteomic analysis on the sperm based on two-dimensional electrophoresis combined with protein identification through mass spectra data. Fifty-six protein spots with constant and relatively high expression levels were selected for protein identification. Among them, 36 were identified (corresponding to 31 proteins), including cytoskeletal proteins, proteins involved in energy supply, protein modifiers, signal regulators, antioxidant proteins, and others. We proposed that these proteins might play important roles in sperm motility, gamete interaction, and oxidation resistance. In particular, we observed several heat shock proteins that were proved to play essential roles in animal sperms. A further ... Text Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Hematology Inorganic Chemistry FOS Chemical sciences Developmental Biology Biological Sciences Earth and Environmental Sciences Evolutionary Biology FOS Biological sciences Molecular Biology Cell Biology Biochemistry Biophysics |
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Hematology Inorganic Chemistry FOS Chemical sciences Developmental Biology Biological Sciences Earth and Environmental Sciences Evolutionary Biology FOS Biological sciences Molecular Biology Cell Biology Biochemistry Biophysics Huang, Xiaohong Liu, Baozhong Huan, Pin The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... |
topic_facet |
Hematology Inorganic Chemistry FOS Chemical sciences Developmental Biology Biological Sciences Earth and Environmental Sciences Evolutionary Biology FOS Biological sciences Molecular Biology Cell Biology Biochemistry Biophysics |
description |
The Pacific oyster Crassostrea gigas is a potential model organism of bivalve mollusks. Comprehensive studies on the proteome of its sperm are necessary to expand our understanding on its reproduction and development, which however are still poor currently. In this study, to improve the situation, we conducted a proteomic analysis on the sperm based on two-dimensional electrophoresis combined with protein identification through mass spectra data. Fifty-six protein spots with constant and relatively high expression levels were selected for protein identification. Among them, 36 were identified (corresponding to 31 proteins), including cytoskeletal proteins, proteins involved in energy supply, protein modifiers, signal regulators, antioxidant proteins, and others. We proposed that these proteins might play important roles in sperm motility, gamete interaction, and oxidation resistance. In particular, we observed several heat shock proteins that were proved to play essential roles in animal sperms. A further ... |
format |
Text |
author |
Huang, Xiaohong Liu, Baozhong Huan, Pin |
author_facet |
Huang, Xiaohong Liu, Baozhong Huan, Pin |
author_sort |
Huang, Xiaohong |
title |
The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... |
title_short |
The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... |
title_full |
The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... |
title_fullStr |
The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... |
title_full_unstemmed |
The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ... |
title_sort |
sperm proteome of the pacific oyster crassostrea gigas and immunolocalization of heat shock proteins ... |
publisher |
Taylor & Francis |
publishDate |
2015 |
url |
https://dx.doi.org/10.6084/m9.figshare.1419594 https://tandf.figshare.com/articles/journal_contribution/The_sperm_proteome_of_the_Pacific_oyster_i_Crassostrea_gigas_i_and_immunolocalization_of_heat_shock_proteins/1419594 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://dx.doi.org/10.1080/07924259.2015.1041654 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.141959410.1080/07924259.2015.1041654 |
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1797581572126277632 |