Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner
Micronutrients (vitamins and minerals) have been less well studied compared to macronutrients (fats, proteins, and carbohydrates) although they play important roles in growth, metabolism, and maintenance of tissues. Hence, there is growing interest to understand the influence of micronutrients acros...
Main Authors: | , , , , , , , |
---|---|
Format: | Other Non-Article Part of Journal/Newspaper |
Language: | unknown |
Published: |
Taylor & Francis
2020
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.13377005.v1 https://tandf.figshare.com/articles/journal_contribution/Micronutrient_supplementation_affects_transcriptional_and_epigenetic_regulation_of_lipid_metabolism_in_a_dose-dependent_manner/13377005/1 |
id |
ftdatacite:10.6084/m9.figshare.13377005.v1 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.13377005.v1 2023-05-15T15:32:54+02:00 Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner Saito, Takaya Whatmore, Paul Taylor, John F. Fernandes, Jorge M.O. Adam, Anne-Catrin Tocher, Douglas R. Espe, Marit Skjærven, Kaja H. 2020 https://dx.doi.org/10.6084/m9.figshare.13377005.v1 https://tandf.figshare.com/articles/journal_contribution/Micronutrient_supplementation_affects_transcriptional_and_epigenetic_regulation_of_lipid_metabolism_in_a_dose-dependent_manner/13377005/1 unknown Taylor & Francis https://dx.doi.org/10.1080/15592294.2020.1859867 https://dx.doi.org/10.6084/m9.figshare.13377005 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer Computational Biology Journal contribution article-journal Text ScholarlyArticle 2020 ftdatacite https://doi.org/10.6084/m9.figshare.13377005.v1 https://doi.org/10.1080/15592294.2020.1859867 https://doi.org/10.6084/m9.figshare.13377005 2022-02-08T15:07:01Z Micronutrients (vitamins and minerals) have been less well studied compared to macronutrients (fats, proteins, and carbohydrates) although they play important roles in growth, metabolism, and maintenance of tissues. Hence, there is growing interest to understand the influence of micronutrients across various aspects in nutritional research. In the last two decades, aquaculture feeds have been shifted to containing more plant-based materials to meet the increasing demand and maintain the sustainability in the industry. A recent whole life cycle feeding trial of Atlantic salmon ( Salmo salar ) with graded levels of micronutrient packages has concluded that the levels of several B-vitamins and microminerals need to be increased from the current recommendation levels for optimal growth and fish welfare when plant-based diets are used. Here, we show the effect of micronutrient supplementation on hepatic transcriptional and epigenetic regulation in a dose dependent manner. . Specifically, our aim is to reveal the mechanisms of altered cell metabolism, which results in improved growth performance by micronutrient surpluses, at gene expression and DNA methylation levels. Our results strongly indicate that micronutrient supplementation suppresses gene expression in lipid metabolism in a dose-dependent manner and broadly affects DNA methylation in cell-adhesion and cell-signalling. In particular, it increases DNA methylation levels on the acetyl-CoA carboxylase alpha promoter in a concentration-dependent manner, which further suggests that acetyl-CoA carboxylase alpha is an upstream epigenetic regulator controlling its downstream lipid biosynthesis activities. This study demonstrates a comprehensive analysis to reveal an important role of micronutrients in lipid metabolism through epigenetic control of gene expression. Other Non-Article Part of Journal/Newspaper Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer Computational Biology |
spellingShingle |
Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer Computational Biology Saito, Takaya Whatmore, Paul Taylor, John F. Fernandes, Jorge M.O. Adam, Anne-Catrin Tocher, Douglas R. Espe, Marit Skjærven, Kaja H. Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
topic_facet |
Biochemistry Cell Biology Genetics FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer Computational Biology |
description |
Micronutrients (vitamins and minerals) have been less well studied compared to macronutrients (fats, proteins, and carbohydrates) although they play important roles in growth, metabolism, and maintenance of tissues. Hence, there is growing interest to understand the influence of micronutrients across various aspects in nutritional research. In the last two decades, aquaculture feeds have been shifted to containing more plant-based materials to meet the increasing demand and maintain the sustainability in the industry. A recent whole life cycle feeding trial of Atlantic salmon ( Salmo salar ) with graded levels of micronutrient packages has concluded that the levels of several B-vitamins and microminerals need to be increased from the current recommendation levels for optimal growth and fish welfare when plant-based diets are used. Here, we show the effect of micronutrient supplementation on hepatic transcriptional and epigenetic regulation in a dose dependent manner. . Specifically, our aim is to reveal the mechanisms of altered cell metabolism, which results in improved growth performance by micronutrient surpluses, at gene expression and DNA methylation levels. Our results strongly indicate that micronutrient supplementation suppresses gene expression in lipid metabolism in a dose-dependent manner and broadly affects DNA methylation in cell-adhesion and cell-signalling. In particular, it increases DNA methylation levels on the acetyl-CoA carboxylase alpha promoter in a concentration-dependent manner, which further suggests that acetyl-CoA carboxylase alpha is an upstream epigenetic regulator controlling its downstream lipid biosynthesis activities. This study demonstrates a comprehensive analysis to reveal an important role of micronutrients in lipid metabolism through epigenetic control of gene expression. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Saito, Takaya Whatmore, Paul Taylor, John F. Fernandes, Jorge M.O. Adam, Anne-Catrin Tocher, Douglas R. Espe, Marit Skjærven, Kaja H. |
author_facet |
Saito, Takaya Whatmore, Paul Taylor, John F. Fernandes, Jorge M.O. Adam, Anne-Catrin Tocher, Douglas R. Espe, Marit Skjærven, Kaja H. |
author_sort |
Saito, Takaya |
title |
Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
title_short |
Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
title_full |
Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
title_fullStr |
Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
title_full_unstemmed |
Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
title_sort |
micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner |
publisher |
Taylor & Francis |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.13377005.v1 https://tandf.figshare.com/articles/journal_contribution/Micronutrient_supplementation_affects_transcriptional_and_epigenetic_regulation_of_lipid_metabolism_in_a_dose-dependent_manner/13377005/1 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1080/15592294.2020.1859867 https://dx.doi.org/10.6084/m9.figshare.13377005 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.13377005.v1 https://doi.org/10.1080/15592294.2020.1859867 https://doi.org/10.6084/m9.figshare.13377005 |
_version_ |
1766363383592910848 |