Polar steroid compounds from the Arctic starfish Asterias microdiscus and their cytotoxic properties against normal and tumor cells in vitro

• Main Authors: Kicha, Alla A., Malyarenko, Timofey V., Kalinovsky, Anatoly I., Popov, Roman S., Malyarenko, Olesya S., Ermakova, Svetlana P., Ivanchina, Natalia V.
• Format: Text
• Language: unknown
• Published: Taylor & Francis 2021
• Subjects: Biophysics; Biochemistry; 29999 Physical Sciences not elsewhere classified; FOS Physical sciences; Cell Biology; Genetics; FOS Biological sciences; Molecular Biology; Pharmacology; 39999 Chemical Sciences not elsewhere classified; FOS Chemical sciences; 69999 Biological Sciences not elsewhere classified; Developmental Biology; Cancer; Arctic
• Online Access: https://dx.doi.org/10.6084/m9.figshare.13122019.v2; https://tandf.figshare.com/articles/journal_contribution/Polar_steroid_compounds_from_the_Arctic_starfish_i_Asterias_microdiscus_i_and_their_cytotoxic_properties_against_normal_and_tumor_cells_i_in_vitro_i_/13122019/2

Two new natural compounds, sulfated polyhydroxysteroid, microdiscusol G ( 1 ), and polyhydroxysteroid bioside, microdiscusoside A ( 2 ), along with eight previously known polar steroid substances 3–10 , were isolated from the Arctic starfish Asterias microdiscus . The structures of 1 and 2 have been elucidated by extensive 1D and 2D NMR spectroscopy and HRESIMS techniques. The 28-sulfooxy-24-methylcholestane side chain in 1 has been found among starfish steroid metabolites for the first time. Steroid saponins 9 and 10 exhibited cytotoxic effects against normal cells JB6 Cl41 and cancer cells HT-29, MDA-MB-231, THP-1, and Raji and effectively suppressed cell proliferation and colony formation of cancer cells HT-29 and MDA-MB-231 in non-toxic concentrations. Compounds 5 , 7 , and 8 were inactive or less active in the same experiments.