The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg
Leishmaniasis is a group of diseases that have limited and high toxic therapeutic options. Herein, we evaluated the antileishmanial potential and cytotoxicity of hexanic extract obtained from the Antarctic brown alga Ascoseira mirabilis using bioguided fractionation against Leishmania amazonensis an...
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ftdatacite:10.6084/m9.figshare.12600861.v1 2023-05-15T13:39:01+02:00 The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg Clementino, Leandro Da Costa Oda, Fernando Bombarda Thaiz Rodrigues Teixeira Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga Dos Hosana Maria Debonsi Graminha, Márcia A. S. 2020 https://dx.doi.org/10.6084/m9.figshare.12600861.v1 https://tandf.figshare.com/articles/The_antileishmanial_activity_of_the_antarctic_brown_alga_i_Ascoseira_mirabilis_i_Skottsberg/12600861/1 unknown Taylor & Francis https://dx.doi.org/10.1080/14786419.2020.1782403 https://dx.doi.org/10.6084/m9.figshare.12600861 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry 29999 Physical Sciences not elsewhere classified FOS Physical sciences Medicine Microbiology FOS Biological sciences Cell Biology Genetics Pharmacology Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Hematology Text article-journal Journal contribution ScholarlyArticle 2020 ftdatacite https://doi.org/10.6084/m9.figshare.12600861.v1 https://doi.org/10.1080/14786419.2020.1782403 https://doi.org/10.6084/m9.figshare.12600861 2021-11-05T12:55:41Z Leishmaniasis is a group of diseases that have limited and high toxic therapeutic options. Herein, we evaluated the antileishmanial potential and cytotoxicity of hexanic extract obtained from the Antarctic brown alga Ascoseira mirabilis using bioguided fractionation against Leishmania amazonensis and murine macrophages, which was fractionated by SPE, yielding seven fractions (F1-F7). The fraction F6 showed good anti-amastigote activity (IC 50 = 73.4 ± 0.4 μg mL −1 ) and low cytotoxicity (CC 50 > 100 μg mL −1 ). Thus, in order to identify the bioactive constituent(s) of F6, the fraction was separated in a semipreparative HPLC, yielding four fractions (F6.1-F6.4). F6.2 was the most bioactive fraction (IC 50 = 66.5 ± 4.5 μg mL −1 ) and GC-MS analyses revealed that the compounds octadecane, propanoic acid, 1-monomyristin and azelaic acid correspond to 61% of its composition. These data show for the first time the antileishmanial potential of the Antarctic alga A. mirabilis . Text Antarc* Antarctic DataCite Metadata Store (German National Library of Science and Technology) Antarctic Skottsberg ENVELOPE(-60.809,-60.809,-63.921,-63.921) The Antarctic |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry 29999 Physical Sciences not elsewhere classified FOS Physical sciences Medicine Microbiology FOS Biological sciences Cell Biology Genetics Pharmacology Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Hematology |
spellingShingle |
Biochemistry 29999 Physical Sciences not elsewhere classified FOS Physical sciences Medicine Microbiology FOS Biological sciences Cell Biology Genetics Pharmacology Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Hematology Clementino, Leandro Da Costa Oda, Fernando Bombarda Thaiz Rodrigues Teixeira Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga Dos Hosana Maria Debonsi Graminha, Márcia A. S. The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
topic_facet |
Biochemistry 29999 Physical Sciences not elsewhere classified FOS Physical sciences Medicine Microbiology FOS Biological sciences Cell Biology Genetics Pharmacology Biotechnology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Hematology |
description |
Leishmaniasis is a group of diseases that have limited and high toxic therapeutic options. Herein, we evaluated the antileishmanial potential and cytotoxicity of hexanic extract obtained from the Antarctic brown alga Ascoseira mirabilis using bioguided fractionation against Leishmania amazonensis and murine macrophages, which was fractionated by SPE, yielding seven fractions (F1-F7). The fraction F6 showed good anti-amastigote activity (IC 50 = 73.4 ± 0.4 μg mL −1 ) and low cytotoxicity (CC 50 > 100 μg mL −1 ). Thus, in order to identify the bioactive constituent(s) of F6, the fraction was separated in a semipreparative HPLC, yielding four fractions (F6.1-F6.4). F6.2 was the most bioactive fraction (IC 50 = 66.5 ± 4.5 μg mL −1 ) and GC-MS analyses revealed that the compounds octadecane, propanoic acid, 1-monomyristin and azelaic acid correspond to 61% of its composition. These data show for the first time the antileishmanial potential of the Antarctic alga A. mirabilis . |
format |
Text |
author |
Clementino, Leandro Da Costa Oda, Fernando Bombarda Thaiz Rodrigues Teixeira Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga Dos Hosana Maria Debonsi Graminha, Márcia A. S. |
author_facet |
Clementino, Leandro Da Costa Oda, Fernando Bombarda Thaiz Rodrigues Teixeira Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga Dos Hosana Maria Debonsi Graminha, Márcia A. S. |
author_sort |
Clementino, Leandro Da Costa |
title |
The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
title_short |
The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
title_full |
The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
title_fullStr |
The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
title_full_unstemmed |
The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
title_sort |
antileishmanial activity of the antarctic brown alga ascoseira mirabilis skottsberg |
publisher |
Taylor & Francis |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.12600861.v1 https://tandf.figshare.com/articles/The_antileishmanial_activity_of_the_antarctic_brown_alga_i_Ascoseira_mirabilis_i_Skottsberg/12600861/1 |
long_lat |
ENVELOPE(-60.809,-60.809,-63.921,-63.921) |
geographic |
Antarctic Skottsberg The Antarctic |
geographic_facet |
Antarctic Skottsberg The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://dx.doi.org/10.1080/14786419.2020.1782403 https://dx.doi.org/10.6084/m9.figshare.12600861 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.12600861.v1 https://doi.org/10.1080/14786419.2020.1782403 https://doi.org/10.6084/m9.figshare.12600861 |
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