Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts
The symbiotic planktonic foraminifera Orbulina universa inhabits open ocean oligotrophic ecosystems where dissolved nutrients are scarce and often limit biological productivity. It has previously been proposed that O. universa meets its nitrogen (N) requirements by preying on zooplankton, and that i...
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ftdatacite:10.6084/m9.figshare.12470273.v1 2023-05-15T18:01:05+02:00 Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts LeKieffre, Charlotte Spero, Howard J. Fehrenbacher, Jennifer S. Russell, Ann D. Haojia Ren Geslin, Emmanuelle Meibom, Anders 2020 https://dx.doi.org/10.6084/m9.figshare.12470273.v1 https://rs.figshare.com/articles/Figure_S5_from_Ammonium_is_the_preferred_source_of_nitrogen_for_planktonic_foraminifer_and_their_dinoflagellate_symbionts/12470273/1 unknown The Royal Society https://dx.doi.org/10.1098/rspb.2020.0620 https://dx.doi.org/10.6084/m9.figshare.12470273 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Microbiology FOS Biological sciences Ecology 40308 Palaeontology incl. Palynology FOS Earth and related environmental sciences Text article-journal Journal contribution ScholarlyArticle 2020 ftdatacite https://doi.org/10.6084/m9.figshare.12470273.v1 https://doi.org/10.1098/rspb.2020.0620 https://doi.org/10.6084/m9.figshare.12470273 2021-11-05T12:55:41Z The symbiotic planktonic foraminifera Orbulina universa inhabits open ocean oligotrophic ecosystems where dissolved nutrients are scarce and often limit biological productivity. It has previously been proposed that O. universa meets its nitrogen (N) requirements by preying on zooplankton, and that its symbiotic dinoflagellates recycle metabolic ‘waste ammonium’ for their N pool. However, these conclusions were derived from bulk 15 N-enrichment experiments and model calculations, and our understanding of N assimilation and exchange between the foraminifer host cell and its symbiotic dinoflagellates remains poorly constrained. Here, we present data from pulse-chase experiments with 13 C-enriched inorganic carbon, 15 N-nitrate and 15 N-ammonium, as well as a 13 C- and 15 N- enriched heterotrophic food source, followed by TEM coupled to NanoSIMS isotopic imaging to visualize and quantify C and N assimilation and translocation in the symbiotic system. High levels of 15 N-labelling were observed in the dinoflagellates and in foraminiferal organelles and cytoplasm after incubation with 15 N-ammonium, indicating efficient ammonium assimilation. Only weak 15 N-assimilation was observed after incubation with 15 N-nitrate. Feeding foraminifers with 13 C- and 15 N-labelled food resulted in dinoflagellates that were labelled with 15 N, thereby confirming the transfer of 15 N-compounds from the digestive vacuoles of the foraminifer to the symbiotic dinoflagellates, likely through recycling of ammonium. These observations are important for N isotope-based paleoceanographic reconstructions, as they show that δ 15 N values recorded in the organic matrix in symbiotic species likely reflect ammonium recycling rather than alternative N sources, such as nitrates. Text Planktonic foraminifera DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Microbiology FOS Biological sciences Ecology 40308 Palaeontology incl. Palynology FOS Earth and related environmental sciences |
spellingShingle |
Microbiology FOS Biological sciences Ecology 40308 Palaeontology incl. Palynology FOS Earth and related environmental sciences LeKieffre, Charlotte Spero, Howard J. Fehrenbacher, Jennifer S. Russell, Ann D. Haojia Ren Geslin, Emmanuelle Meibom, Anders Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
topic_facet |
Microbiology FOS Biological sciences Ecology 40308 Palaeontology incl. Palynology FOS Earth and related environmental sciences |
description |
The symbiotic planktonic foraminifera Orbulina universa inhabits open ocean oligotrophic ecosystems where dissolved nutrients are scarce and often limit biological productivity. It has previously been proposed that O. universa meets its nitrogen (N) requirements by preying on zooplankton, and that its symbiotic dinoflagellates recycle metabolic ‘waste ammonium’ for their N pool. However, these conclusions were derived from bulk 15 N-enrichment experiments and model calculations, and our understanding of N assimilation and exchange between the foraminifer host cell and its symbiotic dinoflagellates remains poorly constrained. Here, we present data from pulse-chase experiments with 13 C-enriched inorganic carbon, 15 N-nitrate and 15 N-ammonium, as well as a 13 C- and 15 N- enriched heterotrophic food source, followed by TEM coupled to NanoSIMS isotopic imaging to visualize and quantify C and N assimilation and translocation in the symbiotic system. High levels of 15 N-labelling were observed in the dinoflagellates and in foraminiferal organelles and cytoplasm after incubation with 15 N-ammonium, indicating efficient ammonium assimilation. Only weak 15 N-assimilation was observed after incubation with 15 N-nitrate. Feeding foraminifers with 13 C- and 15 N-labelled food resulted in dinoflagellates that were labelled with 15 N, thereby confirming the transfer of 15 N-compounds from the digestive vacuoles of the foraminifer to the symbiotic dinoflagellates, likely through recycling of ammonium. These observations are important for N isotope-based paleoceanographic reconstructions, as they show that δ 15 N values recorded in the organic matrix in symbiotic species likely reflect ammonium recycling rather than alternative N sources, such as nitrates. |
format |
Text |
author |
LeKieffre, Charlotte Spero, Howard J. Fehrenbacher, Jennifer S. Russell, Ann D. Haojia Ren Geslin, Emmanuelle Meibom, Anders |
author_facet |
LeKieffre, Charlotte Spero, Howard J. Fehrenbacher, Jennifer S. Russell, Ann D. Haojia Ren Geslin, Emmanuelle Meibom, Anders |
author_sort |
LeKieffre, Charlotte |
title |
Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
title_short |
Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
title_full |
Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
title_fullStr |
Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
title_full_unstemmed |
Figure S5 from Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
title_sort |
figure s5 from ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts |
publisher |
The Royal Society |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.12470273.v1 https://rs.figshare.com/articles/Figure_S5_from_Ammonium_is_the_preferred_source_of_nitrogen_for_planktonic_foraminifer_and_their_dinoflagellate_symbionts/12470273/1 |
genre |
Planktonic foraminifera |
genre_facet |
Planktonic foraminifera |
op_relation |
https://dx.doi.org/10.1098/rspb.2020.0620 https://dx.doi.org/10.6084/m9.figshare.12470273 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.12470273.v1 https://doi.org/10.1098/rspb.2020.0620 https://doi.org/10.6084/m9.figshare.12470273 |
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1766170414967422976 |