Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters
Preventing vibriosis in juvenile cultured Turbot Scophthalmus maximus caused by Vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. Whether the use of probiotics instead of antibiotics in juvenile Turbot is a safer strategy requires more stud...
Main Authors: | , , , |
---|---|
Format: | Text |
Language: | unknown |
Published: |
Taylor & Francis
2014
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.1234092.v1 https://tandf.figshare.com/articles/journal_contribution/Increased_Survival_of_Juvenile_Turbot_i_Scophthalmus_maximus_i_by_Using_Bacteria_Associated_with_Cultured_Oysters/1234092/1 |
id |
ftdatacite:10.6084/m9.figshare.1234092.v1 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.1234092.v1 2023-05-15T18:15:46+02:00 Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters Seguín, Rosa M. Farto Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. 2014 https://dx.doi.org/10.6084/m9.figshare.1234092.v1 https://tandf.figshare.com/articles/journal_contribution/Increased_Survival_of_Juvenile_Turbot_i_Scophthalmus_maximus_i_by_Using_Bacteria_Associated_with_Cultured_Oysters/1234092/1 unknown Taylor & Francis https://dx.doi.org/10.1080/08997659.2014.920734 https://dx.doi.org/10.6084/m9.figshare.1234092 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 110309 Infectious Diseases FOS Health sciences Inorganic Chemistry FOS Chemical sciences Biological Sciences Immunology FOS Clinical medicine Ecology FOS Biological sciences Earth and Environmental Sciences Biotechnology Microbiology Engineering Text article-journal Journal contribution ScholarlyArticle 2014 ftdatacite https://doi.org/10.6084/m9.figshare.1234092.v1 https://doi.org/10.1080/08997659.2014.920734 https://doi.org/10.6084/m9.figshare.1234092 2021-11-05T12:55:41Z Preventing vibriosis in juvenile cultured Turbot Scophthalmus maximus caused by Vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. Whether the use of probiotics instead of antibiotics in juvenile Turbot is a safer strategy requires more study. The antibacterial potential of 148 Vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and Turbot) was analyzed in vitro against V. anguillarum and other pathogens by means of an agar diffusion assay. A wide spectrum of inhibitory activity was shown by 9 strains. Based on their easy phenotypic differentiation from V. anguillarum , we selected two strains (S1 and S2, both isolated from the European flat oyster Ostrea edulis ) for testing in juvenile Turbot (3 g). None of the strains were virulent by intraperitoneal or bath challenges, and all were susceptible to the antibiotics most frequently used in aquaculture. Three different stocks of Turbot, which were assayed separately, were significantly protected from infection with V. anguillarum . The final survival rates of fish treated in mixed challenges with S1 or S2 and V. anguillarum were 44% and 66%, respectively, whereas only 17% of the fish treated with only the pathogenic strain survived. The application of probiotic strains also increased the survival time of juvenile Turbot after infection with V. anguillarum. Both strains persisted in the epidermal mucus layer of the fish for 30 d, and they were not displaced by the pathogen. These data prove the efficacy of using bacteria well adapted to the dynamics of culture production as a way to provide juvenile Turbot immediate protection against infection by V. anguillarum. Moreover, the epidermal mucus sampling was useful for investigating the persistence of both probiotic strains when exposed to the pathogen.Received October 11, 2013; accepted March 10, 2014 Text Scophthalmus maximus Turbot DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
110309 Infectious Diseases FOS Health sciences Inorganic Chemistry FOS Chemical sciences Biological Sciences Immunology FOS Clinical medicine Ecology FOS Biological sciences Earth and Environmental Sciences Biotechnology Microbiology Engineering |
spellingShingle |
110309 Infectious Diseases FOS Health sciences Inorganic Chemistry FOS Chemical sciences Biological Sciences Immunology FOS Clinical medicine Ecology FOS Biological sciences Earth and Environmental Sciences Biotechnology Microbiology Engineering Seguín, Rosa M. Farto Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
topic_facet |
110309 Infectious Diseases FOS Health sciences Inorganic Chemistry FOS Chemical sciences Biological Sciences Immunology FOS Clinical medicine Ecology FOS Biological sciences Earth and Environmental Sciences Biotechnology Microbiology Engineering |
description |
Preventing vibriosis in juvenile cultured Turbot Scophthalmus maximus caused by Vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. Whether the use of probiotics instead of antibiotics in juvenile Turbot is a safer strategy requires more study. The antibacterial potential of 148 Vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and Turbot) was analyzed in vitro against V. anguillarum and other pathogens by means of an agar diffusion assay. A wide spectrum of inhibitory activity was shown by 9 strains. Based on their easy phenotypic differentiation from V. anguillarum , we selected two strains (S1 and S2, both isolated from the European flat oyster Ostrea edulis ) for testing in juvenile Turbot (3 g). None of the strains were virulent by intraperitoneal or bath challenges, and all were susceptible to the antibiotics most frequently used in aquaculture. Three different stocks of Turbot, which were assayed separately, were significantly protected from infection with V. anguillarum . The final survival rates of fish treated in mixed challenges with S1 or S2 and V. anguillarum were 44% and 66%, respectively, whereas only 17% of the fish treated with only the pathogenic strain survived. The application of probiotic strains also increased the survival time of juvenile Turbot after infection with V. anguillarum. Both strains persisted in the epidermal mucus layer of the fish for 30 d, and they were not displaced by the pathogen. These data prove the efficacy of using bacteria well adapted to the dynamics of culture production as a way to provide juvenile Turbot immediate protection against infection by V. anguillarum. Moreover, the epidermal mucus sampling was useful for investigating the persistence of both probiotic strains when exposed to the pathogen.Received October 11, 2013; accepted March 10, 2014 |
format |
Text |
author |
Seguín, Rosa M. Farto Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. |
author_facet |
Seguín, Rosa M. Farto Bermúdez, María Bobo Rivera, Leticia Nieto, Teresa P. |
author_sort |
Seguín, Rosa M. Farto |
title |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_short |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_full |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_fullStr |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_full_unstemmed |
Increased Survival of Juvenile Turbot Scophthalmus maximus by Using Bacteria Associated with Cultured Oysters |
title_sort |
increased survival of juvenile turbot scophthalmus maximus by using bacteria associated with cultured oysters |
publisher |
Taylor & Francis |
publishDate |
2014 |
url |
https://dx.doi.org/10.6084/m9.figshare.1234092.v1 https://tandf.figshare.com/articles/journal_contribution/Increased_Survival_of_Juvenile_Turbot_i_Scophthalmus_maximus_i_by_Using_Bacteria_Associated_with_Cultured_Oysters/1234092/1 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
https://dx.doi.org/10.1080/08997659.2014.920734 https://dx.doi.org/10.6084/m9.figshare.1234092 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.1234092.v1 https://doi.org/10.1080/08997659.2014.920734 https://doi.org/10.6084/m9.figshare.1234092 |
_version_ |
1766188986725826560 |