Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles

• Main Authors: Emam, Mohamed, Katan, Tomer, Caballero-Solares, Albert, Taylor, Richard G., Parrish, Kathleen S., Rise, Matthew L., Parrish, Christopher C.
• Format: Text
• Language: unknown
• Published: The Royal Society 2020
• Subjects: Biochemistry; Ecology; FOS Biological sciences; 60408 Genomics; Atlantic salmon
• Online Access: https://dx.doi.org/10.6084/m9.figshare.12310592.v1; https://rs.figshare.com/articles/Supplementary_material-_Lipid_and_gene_expression_data_and_statistics_from_Interaction_between_i_i_6_and_i_i_3_fatty_acids_of_different_chain_lengths_regulates_Atlantic_salmon_hepatic_gene_expression_and_muscle_fatty_acid_profiles/12310592/1

Atlantic salmon smolts (approx. 20-month old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and EPA + DHA levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) reflected dietary C 18 -polyunsaturated FA; however, muscle EPA % and content (mg g −1 ) were not different in salmon-fed high-ω3 or balanced diets. Muscle DHA % was similar among treatments, while DHA content increased in fish-fed 1.4% EPA + DHA, compared with those fed 0.3–1.0% EPA + DHA combined with high-ω6 FA. Muscle 20 : 3 ω 6 (DGLA) content was highest in those fed high-ω6 with 0.3% EPA + DHA. Hepatic quantitative polymerase chain reaction showed that the monounsaturated FA synthesis-related gene, scdb, was upregulated in fish-fed 1.0% EPA + DHA with high-ω6 compared to those fed 0.3% EPA + DHA. In high-ω3 fed salmon, liver elovl2 transcript levels were higher with 0.3% EPA + DHA than with 1.0% EPA + DHA. In high-ω6 fed fish, elovl2 did not vary with EPA + DHA levels, but it was positively correlated with muscle ARA, 22 : 4 ω 3 and DGLA. These results suggest dietary 18 : 3 ω 3 elongation contributed to maintaining muscle EPA + DHA levels despite a two- to threefold change in dietary proportions, while 18 : 2 ω 6 with 0.3% EPA + DHA increased muscle DGLA more than ARA. Positive correlations between hepatic elovl2 and fabp10a with muscle ω 6: ω 3 and EPA + DHA + ARA, respectively, were confirmed by reanalysing data from a previous salmon trial with lower variations in dietary EPA + DHA and ω 6: ω 3 ratios.This article is part of the XX ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’.