Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles
Atlantic salmon smolts (approx. 20-month old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and EPA + DHA levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) reflected dietary C 18 -polyunsaturated FA; however,...
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ftdatacite:10.6084/m9.figshare.12310592.v1 2023-05-15T15:32:01+02:00 Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. 2020 https://dx.doi.org/10.6084/m9.figshare.12310592.v1 https://rs.figshare.com/articles/Supplementary_material-_Lipid_and_gene_expression_data_and_statistics_from_Interaction_between_i_i_6_and_i_i_3_fatty_acids_of_different_chain_lengths_regulates_Atlantic_salmon_hepatic_gene_expression_and_muscle_fatty_acid_profiles/12310592/1 unknown The Royal Society https://dx.doi.org/10.1098/rstb.2019.0648 https://dx.doi.org/10.6084/m9.figshare.12310592 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Ecology FOS Biological sciences 60408 Genomics Text article-journal Journal contribution ScholarlyArticle 2020 ftdatacite https://doi.org/10.6084/m9.figshare.12310592.v1 https://doi.org/10.1098/rstb.2019.0648 https://doi.org/10.6084/m9.figshare.12310592 2021-11-05T12:55:41Z Atlantic salmon smolts (approx. 20-month old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and EPA + DHA levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) reflected dietary C 18 -polyunsaturated FA; however, muscle EPA % and content (mg g −1 ) were not different in salmon-fed high-ω3 or balanced diets. Muscle DHA % was similar among treatments, while DHA content increased in fish-fed 1.4% EPA + DHA, compared with those fed 0.3–1.0% EPA + DHA combined with high-ω6 FA. Muscle 20 : 3 ω 6 (DGLA) content was highest in those fed high-ω6 with 0.3% EPA + DHA. Hepatic quantitative polymerase chain reaction showed that the monounsaturated FA synthesis-related gene, scdb, was upregulated in fish-fed 1.0% EPA + DHA with high-ω6 compared to those fed 0.3% EPA + DHA. In high-ω3 fed salmon, liver elovl2 transcript levels were higher with 0.3% EPA + DHA than with 1.0% EPA + DHA. In high-ω6 fed fish, elovl2 did not vary with EPA + DHA levels, but it was positively correlated with muscle ARA, 22 : 4 ω 3 and DGLA. These results suggest dietary 18 : 3 ω 3 elongation contributed to maintaining muscle EPA + DHA levels despite a two- to threefold change in dietary proportions, while 18 : 2 ω 6 with 0.3% EPA + DHA increased muscle DGLA more than ARA. Positive correlations between hepatic elovl2 and fabp10a with muscle ω 6: ω 3 and EPA + DHA + ARA, respectively, were confirmed by reanalysing data from a previous salmon trial with lower variations in dietary EPA + DHA and ω 6: ω 3 ratios.This article is part of the XX ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’. Text Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Ecology FOS Biological sciences 60408 Genomics |
spellingShingle |
Biochemistry Ecology FOS Biological sciences 60408 Genomics Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
topic_facet |
Biochemistry Ecology FOS Biological sciences 60408 Genomics |
description |
Atlantic salmon smolts (approx. 20-month old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and EPA + DHA levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) reflected dietary C 18 -polyunsaturated FA; however, muscle EPA % and content (mg g −1 ) were not different in salmon-fed high-ω3 or balanced diets. Muscle DHA % was similar among treatments, while DHA content increased in fish-fed 1.4% EPA + DHA, compared with those fed 0.3–1.0% EPA + DHA combined with high-ω6 FA. Muscle 20 : 3 ω 6 (DGLA) content was highest in those fed high-ω6 with 0.3% EPA + DHA. Hepatic quantitative polymerase chain reaction showed that the monounsaturated FA synthesis-related gene, scdb, was upregulated in fish-fed 1.0% EPA + DHA with high-ω6 compared to those fed 0.3% EPA + DHA. In high-ω3 fed salmon, liver elovl2 transcript levels were higher with 0.3% EPA + DHA than with 1.0% EPA + DHA. In high-ω6 fed fish, elovl2 did not vary with EPA + DHA levels, but it was positively correlated with muscle ARA, 22 : 4 ω 3 and DGLA. These results suggest dietary 18 : 3 ω 3 elongation contributed to maintaining muscle EPA + DHA levels despite a two- to threefold change in dietary proportions, while 18 : 2 ω 6 with 0.3% EPA + DHA increased muscle DGLA more than ARA. Positive correlations between hepatic elovl2 and fabp10a with muscle ω 6: ω 3 and EPA + DHA + ARA, respectively, were confirmed by reanalysing data from a previous salmon trial with lower variations in dietary EPA + DHA and ω 6: ω 3 ratios.This article is part of the XX ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’. |
format |
Text |
author |
Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. |
author_facet |
Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. |
author_sort |
Emam, Mohamed |
title |
Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_short |
Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_full |
Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_fullStr |
Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_full_unstemmed |
Supplementary material- Lipid and gene expression data and statistics from Interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_sort |
supplementary material- lipid and gene expression data and statistics from interaction between ω 6 and ω 3 fatty acids of different chain lengths regulates atlantic salmon hepatic gene expression and muscle fatty acid profiles |
publisher |
The Royal Society |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.12310592.v1 https://rs.figshare.com/articles/Supplementary_material-_Lipid_and_gene_expression_data_and_statistics_from_Interaction_between_i_i_6_and_i_i_3_fatty_acids_of_different_chain_lengths_regulates_Atlantic_salmon_hepatic_gene_expression_and_muscle_fatty_acid_profiles/12310592/1 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1098/rstb.2019.0648 https://dx.doi.org/10.6084/m9.figshare.12310592 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.12310592.v1 https://doi.org/10.1098/rstb.2019.0648 https://doi.org/10.6084/m9.figshare.12310592 |
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