Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity
During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (...
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ftdatacite:10.6084/m9.figshare.11468109.v1 2023-05-15T14:28:54+02:00 Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity Hagestad, Ole Christian Andersen, Jeanette H. Altermark, Bjørn Hansen, Espen Rämä, Teppo 2019 https://dx.doi.org/10.6084/m9.figshare.11468109.v1 https://tandf.figshare.com/articles/dataset/Cultivable_marine_fungi_from_the_Arctic_Archipelago_of_Svalbard_and_their_antibacterial_activity/11468109/1 unknown Taylor & Francis https://dx.doi.org/10.1080/21501203.2019.1708492 https://dx.doi.org/10.6084/m9.figshare.11468109 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Microbiology FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences dataset Dataset 2019 ftdatacite https://doi.org/10.6084/m9.figshare.11468109.v1 https://doi.org/10.1080/21501203.2019.1708492 https://doi.org/10.6084/m9.figshare.11468109 2021-11-05T12:55:41Z During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73–80 °N, 18–31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98% similarity. The taxonomic analysis showed that 17 OTUs belonged to Ascomycota, one to Basidiomycota, two to Mucoromycota and two were fungal-like organisms. The nuc rDNA V1-V5 regions of 18S (18S) and D1-D3 regions of 28S (28S) were sequenced from representative isolates of each OTU for comparison to GenBank sequences. Isolates of Lulworthiales and Eurotiales were the most abundant, with seven isolates each. Among the 22 OTUs, nine represent potentially undescribed species based on low similarity to GenBank sequences and 10 isolates showed inhibitory activity against Gram-positive bacteria in an agar diffusion plug assay. These results show promise for the Arctic region as asource of novel marine fungi with the ability to produce bioactive secondary metabolites with antibacterial properties. Dataset Arctic Archipelago Arctic Svalbard DataCite Metadata Store (German National Library of Science and Technology) Arctic Svalbard Svalbard Archipelago |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Microbiology FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
spellingShingle |
Microbiology FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Hagestad, Ole Christian Andersen, Jeanette H. Altermark, Bjørn Hansen, Espen Rämä, Teppo Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
topic_facet |
Microbiology FOS Biological sciences Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73–80 °N, 18–31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98% similarity. The taxonomic analysis showed that 17 OTUs belonged to Ascomycota, one to Basidiomycota, two to Mucoromycota and two were fungal-like organisms. The nuc rDNA V1-V5 regions of 18S (18S) and D1-D3 regions of 28S (28S) were sequenced from representative isolates of each OTU for comparison to GenBank sequences. Isolates of Lulworthiales and Eurotiales were the most abundant, with seven isolates each. Among the 22 OTUs, nine represent potentially undescribed species based on low similarity to GenBank sequences and 10 isolates showed inhibitory activity against Gram-positive bacteria in an agar diffusion plug assay. These results show promise for the Arctic region as asource of novel marine fungi with the ability to produce bioactive secondary metabolites with antibacterial properties. |
format |
Dataset |
author |
Hagestad, Ole Christian Andersen, Jeanette H. Altermark, Bjørn Hansen, Espen Rämä, Teppo |
author_facet |
Hagestad, Ole Christian Andersen, Jeanette H. Altermark, Bjørn Hansen, Espen Rämä, Teppo |
author_sort |
Hagestad, Ole Christian |
title |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_short |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_full |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_fullStr |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_full_unstemmed |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_sort |
cultivable marine fungi from the arctic archipelago of svalbard and their antibacterial activity |
publisher |
Taylor & Francis |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.11468109.v1 https://tandf.figshare.com/articles/dataset/Cultivable_marine_fungi_from_the_Arctic_Archipelago_of_Svalbard_and_their_antibacterial_activity/11468109/1 |
geographic |
Arctic Svalbard Svalbard Archipelago |
geographic_facet |
Arctic Svalbard Svalbard Archipelago |
genre |
Arctic Archipelago Arctic Svalbard |
genre_facet |
Arctic Archipelago Arctic Svalbard |
op_relation |
https://dx.doi.org/10.1080/21501203.2019.1708492 https://dx.doi.org/10.6084/m9.figshare.11468109 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.11468109.v1 https://doi.org/10.1080/21501203.2019.1708492 https://doi.org/10.6084/m9.figshare.11468109 |
_version_ |
1766303033745997824 |