Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface
The surface interactions between gold nanoparticles (AuNPs) and lipase from Candida antarctica fraction B (CALB) were investigated at macromolecular and colloidal length-scales. In order to elucidate the reciprocal effect of the interactions on the individual component’s properties, CALB was either...
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ftdatacite:10.6084/m9.figshare.10026242 2023-05-15T13:31:55+02:00 Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface Barros, Heloise R. De Santos, Mary C. Barbosa, Leandro R. S. Piovan, Leandro Izabel C. Riegel-Vidotti 2019 https://dx.doi.org/10.6084/m9.figshare.10026242 https://scielo.figshare.com/articles/Physicochemical_Study_of_the_Interaction_between_Gold_Nanoparticles_and_Lipase_from_Candida_sp_CALB_Insights_into_the_Nano-Bio_Interface/10026242 unknown SciELO journals https://dx.doi.org/10.21577/0103-5053.20190127 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 30699 Physical Chemistry not elsewhere classified FOS Chemical sciences dataset Dataset 2019 ftdatacite https://doi.org/10.6084/m9.figshare.10026242 https://doi.org/10.21577/0103-5053.20190127 2021-11-05T12:55:41Z The surface interactions between gold nanoparticles (AuNPs) and lipase from Candida antarctica fraction B (CALB) were investigated at macromolecular and colloidal length-scales. In order to elucidate the reciprocal effect of the interactions on the individual component’s properties, CALB was either added during the synthesis of AuNPs or added to pre-synthesized AuNPs. In both cases, it was observed that the AuNPs are spherical and stable and, by fluorescence and circular dichroism spectroscopies, changes were observed in the secondary and tertiary structure of CALB, that were dependent on the AuNPs concentration. Nevertheless, the catalytic activity of CALB was maintained, although at a lower percentage (≥ 80%), thus new bio-functionalities were inserted into AuNPs upon interaction with CALB. Using simple and straightforward approaches and state-of-the-art techniques important knowledge about CALB/AuNPs bioconjugates was gained, thus contributing to the development of new nano-biomaterials and for the safe use of AuNPs in biomedical applications. Dataset Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
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unknown |
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30699 Physical Chemistry not elsewhere classified FOS Chemical sciences |
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30699 Physical Chemistry not elsewhere classified FOS Chemical sciences Barros, Heloise R. De Santos, Mary C. Barbosa, Leandro R. S. Piovan, Leandro Izabel C. Riegel-Vidotti Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface |
topic_facet |
30699 Physical Chemistry not elsewhere classified FOS Chemical sciences |
description |
The surface interactions between gold nanoparticles (AuNPs) and lipase from Candida antarctica fraction B (CALB) were investigated at macromolecular and colloidal length-scales. In order to elucidate the reciprocal effect of the interactions on the individual component’s properties, CALB was either added during the synthesis of AuNPs or added to pre-synthesized AuNPs. In both cases, it was observed that the AuNPs are spherical and stable and, by fluorescence and circular dichroism spectroscopies, changes were observed in the secondary and tertiary structure of CALB, that were dependent on the AuNPs concentration. Nevertheless, the catalytic activity of CALB was maintained, although at a lower percentage (≥ 80%), thus new bio-functionalities were inserted into AuNPs upon interaction with CALB. Using simple and straightforward approaches and state-of-the-art techniques important knowledge about CALB/AuNPs bioconjugates was gained, thus contributing to the development of new nano-biomaterials and for the safe use of AuNPs in biomedical applications. |
format |
Dataset |
author |
Barros, Heloise R. De Santos, Mary C. Barbosa, Leandro R. S. Piovan, Leandro Izabel C. Riegel-Vidotti |
author_facet |
Barros, Heloise R. De Santos, Mary C. Barbosa, Leandro R. S. Piovan, Leandro Izabel C. Riegel-Vidotti |
author_sort |
Barros, Heloise R. De |
title |
Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface |
title_short |
Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface |
title_full |
Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface |
title_fullStr |
Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface |
title_full_unstemmed |
Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface |
title_sort |
physicochemical study of the interaction between gold nanoparticles and lipase from candida sp. (calb): insights into the nano-bio interface |
publisher |
SciELO journals |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.10026242 https://scielo.figshare.com/articles/Physicochemical_Study_of_the_Interaction_between_Gold_Nanoparticles_and_Lipase_from_Candida_sp_CALB_Insights_into_the_Nano-Bio_Interface/10026242 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.21577/0103-5053.20190127 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.10026242 https://doi.org/10.21577/0103-5053.20190127 |
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1766022447170060288 |