CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods...
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2022
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Online Access: | https://dx.doi.org/10.6075/j0zw1kcf https://library.ucsd.edu/dc/object/bb6187156w |
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ftdatacite:10.6075/j0zw1kcf 2024-02-04T10:03:43+01:00 CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... Buckles, Thomas 2022 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0zw1kcf https://library.ucsd.edu/dc/object/bb6187156w unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil51976 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Mus musculus Cell polarity establishment and maintenance RAW 264.7 TIB-71 Whole cell Dataset dataset 2022 ftdatacite https://doi.org/10.6075/j0zw1kcf10.7295/w9cil5197610.6075/j0r20zxn 2024-01-05T01:28:39Z ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK" 5. The 16 bit image will typically look dark and will require ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
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unknown |
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Mus musculus Cell polarity establishment and maintenance RAW 264.7 TIB-71 Whole cell |
spellingShingle |
Mus musculus Cell polarity establishment and maintenance RAW 264.7 TIB-71 Whole cell Buckles, Thomas CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
topic_facet |
Mus musculus Cell polarity establishment and maintenance RAW 264.7 TIB-71 Whole cell |
description |
~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK" 5. The 16 bit image will typically look dark and will require ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... |
format |
Dataset |
author |
Buckles, Thomas |
author_facet |
Buckles, Thomas |
author_sort |
Buckles, Thomas |
title |
CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_short |
CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full |
CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_fullStr |
CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full_unstemmed |
CIL: 51976, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_sort |
cil: 51976, mus musculus, raw 264.7 tib-71. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2022 |
url |
https://dx.doi.org/10.6075/j0zw1kcf https://library.ucsd.edu/dc/object/bb6187156w |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil51976 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6075/j0zw1kcf10.7295/w9cil5197610.6075/j0r20zxn |
_version_ |
1789971346993709056 |