CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ...
Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization m...
Main Authors: | , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
UC San Diego Library Digital Collections
2021
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6075/j0wq0357 https://library.ucsd.edu/dc/object/bb5502411p |
id |
ftdatacite:10.6075/j0wq0357 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6075/j0wq0357 2024-02-04T09:59:28+01:00 CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... Zaritsky, Assaf Kaplan, Doron 2021 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0wq0357 https://library.ucsd.edu/dc/object/bb5502411p unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil44506 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Canis lupus familiaris kidney epithelial cell wound healing, spreading of epidermal cells MDCK-II cell migration Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0wq035710.7295/w9cil4450610.6075/j0r20zxn 2024-01-05T01:28:39Z Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Madin-Darby Canine Kidney (MDCK) epithelial cells expressing YFP-membrane were maintained in DMEM supplemented with 5% fetal FCS in a 37°C, 5% CO2 incubator. Wound healing assay: Cells were grown to 90% confluence in 24-well plates. Prior to scratching, the cells were starved by changing the medium to DMEM plus 0.1% FCS (starvation medium) for 24 hours. The medium was then changed to either fresh starvation medium (control), or starvation medium with 80 ng ml-1 HGF/SF for an additional 2 hours. A scratch of approximately 300 μm in width was generated using a 200 μl tip. The plate was subjected to time lapse microscopy in a stage incubator (OKOLAB, Italy) on a computer-controlled motorized stage of a confocal microscope (CLSM-510, Carl Zeiss, Germany), used in non-confocal DIC mode, with a 10x (NA 0.30) objective. Image acquisition was initiated 2 hours post scratching. Images were acquired every 15.7 minutes for 15 hours. The coordinates of each scratch were predefined, and a macro that repetitively ... Dataset Canis lupus DataCite Metadata Store (German National Library of Science and Technology) Darby ENVELOPE(162.217,162.217,-77.667,-77.667) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Canis lupus familiaris kidney epithelial cell wound healing, spreading of epidermal cells MDCK-II cell migration |
spellingShingle |
Canis lupus familiaris kidney epithelial cell wound healing, spreading of epidermal cells MDCK-II cell migration Zaritsky, Assaf Kaplan, Doron CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... |
topic_facet |
Canis lupus familiaris kidney epithelial cell wound healing, spreading of epidermal cells MDCK-II cell migration |
description |
Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Madin-Darby Canine Kidney (MDCK) epithelial cells expressing YFP-membrane were maintained in DMEM supplemented with 5% fetal FCS in a 37°C, 5% CO2 incubator. Wound healing assay: Cells were grown to 90% confluence in 24-well plates. Prior to scratching, the cells were starved by changing the medium to DMEM plus 0.1% FCS (starvation medium) for 24 hours. The medium was then changed to either fresh starvation medium (control), or starvation medium with 80 ng ml-1 HGF/SF for an additional 2 hours. A scratch of approximately 300 μm in width was generated using a 200 μl tip. The plate was subjected to time lapse microscopy in a stage incubator (OKOLAB, Italy) on a computer-controlled motorized stage of a confocal microscope (CLSM-510, Carl Zeiss, Germany), used in non-confocal DIC mode, with a 10x (NA 0.30) objective. Image acquisition was initiated 2 hours post scratching. Images were acquired every 15.7 minutes for 15 hours. The coordinates of each scratch were predefined, and a macro that repetitively ... |
format |
Dataset |
author |
Zaritsky, Assaf Kaplan, Doron |
author_facet |
Zaritsky, Assaf Kaplan, Doron |
author_sort |
Zaritsky, Assaf |
title |
CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... |
title_short |
CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... |
title_full |
CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... |
title_fullStr |
CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:44506, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ... |
title_sort |
cil:44506, canis lupus familiaris, kidney epithelial cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0wq0357 https://library.ucsd.edu/dc/object/bb5502411p |
long_lat |
ENVELOPE(162.217,162.217,-77.667,-77.667) |
geographic |
Darby |
geographic_facet |
Darby |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
https://dx.doi.org/10.7295/w9cil44506 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j0wq035710.7295/w9cil4450610.6075/j0r20zxn |
_version_ |
1789964298194255872 |