CIL:39975, Phaeocystis antarctica, algae. In Cell Image Library ...

• Main Authors: Moisan, Tiffany, Sosinsky, Gina, Buitenhuys, Casey, Ellisman, Mark
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: thylakoid; algae; photosynthetic electron transport chain; thylakoid membrane organization; Phaeocystis antarctica; Chloroplast; Antarc*; Antarctica; antartic*
• Online Access: https://dx.doi.org/10.6075/j0wm1dhs; https://library.ucsd.edu/dc/object/bb9120557z

Culture conditions. Cultures of colonial P. antarctica (CCMP 1374) were grown semi-continuously for 5-8 generations in f/2 medium (Guillard and Ryther 1962) under continuous blue light at 4°C at irradiances of 14 and 259 µmol quanta m-2 s-1. Specific growth rate. Specific growth rate was estimated by a linear regression of loge transformed daily determinations of in vivo fluorescence intensity (n=2) measured with a Turner Model 10 fluorometer. Sample preparation for electron microscopy. P. antarctica colonies were fixed on ice with a 2% glutaraldehyde and 1.3% osmium tetroxide solution for 30 minutes and rinsed in distilled water. Cells were dehydrated through a series of ethanol: water washes (25:75, 50:50, 75:25, 95:5), three 100% ethanol washes and finally through three washes of 100% acetone. Cells were pelleted and fixed in an Epon resin. The fixation process lends itself to a breakup of the colonial matrix and we were able to examine P. antarctica individual colonial cells using electron tomography. ... : Computed slice through a tomographic volume of a chloroplast from Phaeocystis antartica, a blue green algae, grown under low light conditions. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Moisan, T., Ellisman, M. H., Buitenhuys, C.W., Sosinsky, G. E., (2006) Differences in Chloroplast Ultrastructure of Phaeocystis antarctica in High and Low Light Conditions, Marine Biology, 149 (6) 1281-1290. ...