CIL:11932, Taricha granulosa, epithelial cell of lung. In Cell Image Library ...

• Main Authors: Salmon, Wendy C., Adams, Michael C., Waterman-Storer, Clare M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: actin cytoskeleton; actin filament polymerization; Taricha granulosa; epithelial cell of lung; actin filament depolymerization; Orca
• Online Access: https://dx.doi.org/10.6075/j0w66jh6; https://library.ucsd.edu/dc/object/bb84708060

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: background subtraction, 3X3 low pass filter, unsharp mask Data qualification: Processed;spatialmeasurements;intensitiesquantitation ... : Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin. At the leading edge and throughout the lamellipodium, f-actin speckles continuously appeared and moved towards the cell center. As speckles approached the junction between the lamellipodium and lamellum they usually disappeared, F-actin underwent slower retrograde flow in the lamellum, and F-actin generally moved forward in the cell body. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50 mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10 s intervals. Images were processed as ...