CIL:11932, Taricha granulosa, epithelial cell of lung. In Cell Image Library ...
Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods:...
Main Authors: | , , |
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Format: | Dataset |
Language: | unknown |
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UC San Diego Library Digital Collections
2021
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Online Access: | https://dx.doi.org/10.6075/j0w66jh6 https://library.ucsd.edu/dc/object/bb84708060 |
Summary: | Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: background subtraction, 3X3 low pass filter, unsharp mask Data qualification: Processed;spatialmeasurements;intensitiesquantitation ... : Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin. At the leading edge and throughout the lamellipodium, f-actin speckles continuously appeared and moved towards the cell center. As speckles approached the junction between the lamellipodium and lamellum they usually disappeared, F-actin underwent slower retrograde flow in the lamellum, and F-actin generally moved forward in the cell body. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50 mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10 s intervals. Images were processed as ... |
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