CIL:44512, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ...

• Main Authors: Zaritsky, Assaf, Kaplan, Doron
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: cell migration; kidney epithelial cell; MDCK-II; wound healing, spreading of epidermal cells; Canis lupus familiaris; Darby; Canis lupus
• Online Access: https://dx.doi.org/10.6075/j0vm4bm4; https://library.ucsd.edu/dc/object/bb7106523j

Movie prepared from time series light microscopy images illustrating a wound healing assay. A monolayer of MDCK (Madin-Darby Canine Kidney) epithelial cells is scratched to create a 'wound' about 300 micrometers in width, after which the growth of the cells to fill the wound is monitored by recording images over the course of 15h. This movie is part of a group of 12 that includes control cells (CIL:44501, 502, 506, 507, 508) and after addition of Hepatocyte Growth Factor /Scatter Factor (HGF/SF) to activate HGF/SF-Met signaling (CIL:44503, 504, 505, 509, 510). Also in the group are two movies, one control (CIL:44511 created from CIL:44501), and one HGF/SF treated (this image, created from CIL:44512). For additional wound healing assays see CIL:43401 and images grouped with it. ... : Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Data qualification: Processed ...