CIL:41466, Sus scrofa domestica, epithelial cell. In Cell Image Library ...

• Main Author: Wadsworth, Patricia
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: mitosis; Sus scrofa domestica; spindle; epithelial cell; LLC-PK1; actin cytoskeleton; Olympus; Orca
• Online Access: https://dx.doi.org/10.6075/j0vm4b3d; https://library.ucsd.edu/dc/object/bb7105588m

Confocal image of a mitotic spindle in a dividing cell. The spindle is shown in yellow and the surrounding actin cytoskeleton is in blue. Images were acquired using a 100X NA 1.4 phase objective lens and a Perkin Elmer spinning disc confocal scan head. A Hammamatsu Orca ER cooled CCD camera was used to collect images. Image collection was driven using Metamorph software. Complete Z-series of images were collected and deconvolved using AutoQuant software. The image is a maximum intensity projection of a subset of the entire Z-stack; the same subset of the Z-stack is used in each channel. Sixth Prize, 2007 Olympus BioScapes Digital Imaging Competition. ... : Preparation: glutaraldehyde fixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: confocal microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of epitope Visualization methods: phalloidin; primary antibody plus labeled secondary antibody; Fluorescein (FITC); Rhodamine Processing history: deconvolution; maximum projection ...