CIL:13159, Mus musculus, fibroblast. In Cell Image Library ...

• Main Authors: Doyle, Andrew D., Wang, Francis W., Matsumoto, Kazue, Yamada, Kenneth M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: fibroblast; cell; Mus musculus; cell migration; substrate-dependent cell migration, cell attachment to substrate; NIH/3T3; Orca
• Online Access: https://dx.doi.org/10.6075/j0tt4png; https://library.ucsd.edu/dc/object/bb8880398w

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: phase contrast microscopy Parameter imaged: elastic scattering of photons Source of contrast: differences in amount of elastic light scattering Visualization methods: visualization of contiguous regions Processing history: custom-made smoothing, sharpening, and convolution filters using MetaMorph software Data qualification: Processed ... : Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 4 min and shown at 15 frames per second. ...